Geranyl Acetate Synthesis in a Packed-Bed Reactor Catalyzed by Novozym in Supercritical Carbon Dioxide and in Supercritical Ethane

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Abstract

The esterification reaction of geraniol with acetic acid (100 mM/100 mM) catalyzed by immobilized Candida antarctica lipase B (Novozym 435) was studied in supercritical carbon dioxide (sc-CO(2)) and in sc-ethane in a packed-bed reactor (PBR). In sc-CO(2) it was easy to adjust the water activity (a(w)) in the reaction mixture to levels leading to good enzyme performance. Enzyme stability was high and steady-state conversions could be achieved that exceeded the largest conversions measured in batch stirred-tank reactors (BSTRs), which is probably due to the lower a(w) levels achieved in the PBR. In sc-ethane, where the solubility of water is lower, high steady-state conversions could be attained only by preventing the accumulation on the enzyme bed of the water produced during reaction. The kinetic parameters for the reaction in sc-CO(2) were determined using previously published data obtained in a BSTR, and a model was developed for the PBR that included those kinetic parameters. This model was able to predict with reasonable accuracy the behavior of the PBR. Slight differences were observed for some operating regions, probably due to the influence of aw in the activity of the enzyme, which is not included in the model.
Original languageUnknown
Pages (from-to)1938-1946
JournalIndustrial & Engineering Chemistry Research
Volume50
Issue number4
DOIs
Publication statusPublished - 1 Jan 2011

Keywords

    Cite this

    @article{b1d5854cffaa4df1a41c8aa7736bfbca,
    title = "Geranyl Acetate Synthesis in a Packed-Bed Reactor Catalyzed by Novozym in Supercritical Carbon Dioxide and in Supercritical Ethane",
    abstract = "The esterification reaction of geraniol with acetic acid (100 mM/100 mM) catalyzed by immobilized Candida antarctica lipase B (Novozym 435) was studied in supercritical carbon dioxide (sc-CO(2)) and in sc-ethane in a packed-bed reactor (PBR). In sc-CO(2) it was easy to adjust the water activity (a(w)) in the reaction mixture to levels leading to good enzyme performance. Enzyme stability was high and steady-state conversions could be achieved that exceeded the largest conversions measured in batch stirred-tank reactors (BSTRs), which is probably due to the lower a(w) levels achieved in the PBR. In sc-ethane, where the solubility of water is lower, high steady-state conversions could be attained only by preventing the accumulation on the enzyme bed of the water produced during reaction. The kinetic parameters for the reaction in sc-CO(2) were determined using previously published data obtained in a BSTR, and a model was developed for the PBR that included those kinetic parameters. This model was able to predict with reasonable accuracy the behavior of the PBR. Slight differences were observed for some operating regions, probably due to the influence of aw in the activity of the enzyme, which is not included in the model.",
    keywords = "CONTINUOUS OPERATION, ORGANIC-SOLVENTS, IMMOBILIZED ENZYME, LIPASE, ADSORPTIVE CONTROL, ESTER SYNTHESIS, IONIC LIQUIDS, N-HEXANE, NONCONVENTIONAL MEDIA, WATER ACTIVITY CONTROL",
    author = "Barreiros, {Susana Filipe}",
    year = "2011",
    month = "1",
    day = "1",
    doi = "10.1021/ie101489j",
    language = "Unknown",
    volume = "50",
    pages = "1938--1946",
    journal = "Industrial & Engineering Chemistry Research",
    issn = "0888-5885",
    publisher = "AMER CHEMICAL SOC",
    number = "4",

    }

    TY - JOUR

    T1 - Geranyl Acetate Synthesis in a Packed-Bed Reactor Catalyzed by Novozym in Supercritical Carbon Dioxide and in Supercritical Ethane

    AU - Barreiros, Susana Filipe

    PY - 2011/1/1

    Y1 - 2011/1/1

    N2 - The esterification reaction of geraniol with acetic acid (100 mM/100 mM) catalyzed by immobilized Candida antarctica lipase B (Novozym 435) was studied in supercritical carbon dioxide (sc-CO(2)) and in sc-ethane in a packed-bed reactor (PBR). In sc-CO(2) it was easy to adjust the water activity (a(w)) in the reaction mixture to levels leading to good enzyme performance. Enzyme stability was high and steady-state conversions could be achieved that exceeded the largest conversions measured in batch stirred-tank reactors (BSTRs), which is probably due to the lower a(w) levels achieved in the PBR. In sc-ethane, where the solubility of water is lower, high steady-state conversions could be attained only by preventing the accumulation on the enzyme bed of the water produced during reaction. The kinetic parameters for the reaction in sc-CO(2) were determined using previously published data obtained in a BSTR, and a model was developed for the PBR that included those kinetic parameters. This model was able to predict with reasonable accuracy the behavior of the PBR. Slight differences were observed for some operating regions, probably due to the influence of aw in the activity of the enzyme, which is not included in the model.

    AB - The esterification reaction of geraniol with acetic acid (100 mM/100 mM) catalyzed by immobilized Candida antarctica lipase B (Novozym 435) was studied in supercritical carbon dioxide (sc-CO(2)) and in sc-ethane in a packed-bed reactor (PBR). In sc-CO(2) it was easy to adjust the water activity (a(w)) in the reaction mixture to levels leading to good enzyme performance. Enzyme stability was high and steady-state conversions could be achieved that exceeded the largest conversions measured in batch stirred-tank reactors (BSTRs), which is probably due to the lower a(w) levels achieved in the PBR. In sc-ethane, where the solubility of water is lower, high steady-state conversions could be attained only by preventing the accumulation on the enzyme bed of the water produced during reaction. The kinetic parameters for the reaction in sc-CO(2) were determined using previously published data obtained in a BSTR, and a model was developed for the PBR that included those kinetic parameters. This model was able to predict with reasonable accuracy the behavior of the PBR. Slight differences were observed for some operating regions, probably due to the influence of aw in the activity of the enzyme, which is not included in the model.

    KW - CONTINUOUS OPERATION

    KW - ORGANIC-SOLVENTS

    KW - IMMOBILIZED ENZYME

    KW - LIPASE

    KW - ADSORPTIVE CONTROL

    KW - ESTER SYNTHESIS

    KW - IONIC LIQUIDS

    KW - N-HEXANE

    KW - NONCONVENTIONAL MEDIA

    KW - WATER ACTIVITY CONTROL

    U2 - 10.1021/ie101489j

    DO - 10.1021/ie101489j

    M3 - Article

    VL - 50

    SP - 1938

    EP - 1946

    JO - Industrial & Engineering Chemistry Research

    JF - Industrial & Engineering Chemistry Research

    SN - 0888-5885

    IS - 4

    ER -