Genotoxicity of quercetin in the micronucleus assay in mouse bone marrow erythrocytes, human lymphocytes, V79 cell line and identification of kinetochore-containing (CREST staining) micronuclei in human lymphocytes

Helena Caria, Teresa Chaveca, António Laires, José Rueff

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Quercetin, a mutagenic flavonoid widely distributed in edible plants, was studied for the induction of micronuclei (MN). We have carried out the MN assay in bone marrow polychromatic erythrocytes in mice, in cytokinesis-blocked human lymphocytes and in cytokinesis-blocked V79 cells. MN assay in vitro was performed in the presence and in the absence of S9. To further extend the study, an antikinetochore antibody (CREST staining) was used to distinguish MN containing whole chromosomes (kinetochore positive) from those containing acentric fragments (kinetochore negative). When tested in vivo quercetin failed to induce micronuclei, a result which is in agreement with other published reports. When tested in vitro in V79 cells quercetin clearly induces micronuclei in the absence of S9 and also in the presence of S9 for the highest dose used. When tested in vitro in human lymphocytes quercetin shows a significant induction of micronuclei in the absence and in the presence of S9. The presence of s9 compared to its absence is not significant for any of the systems used. Both in the presence and absence of S9, quercetin appears to behave as a clastogenic agent in human lymphocytes inducing a significant majority of kinetochore-negative MN.

Original languageEnglish
Pages (from-to)85-94
Number of pages10
JournalMutation Research/Genetic Toxicology
Volume343
Issue number2-3
DOIs
Publication statusPublished - 1 Jan 1995

Fingerprint

Kinetochores
Micronucleus Tests
Lymphocytes
Quercetin
Assays
Bone
Erythrocytes
Bone Marrow
Cells
Staining and Labeling
Cell Line
Cytokinesis
Edible Plants
Chromosomes
Flavonoids
Antibodies
In Vitro Techniques

Keywords

  • Antikinetochore antibody
  • Human lymphocyte
  • Micronucleus
  • Mouse bone marrow
  • Quercetin
  • V79 cell line

Cite this

@article{caf4290e26c64bf68f220943cbeb82cd,
title = "Genotoxicity of quercetin in the micronucleus assay in mouse bone marrow erythrocytes, human lymphocytes, V79 cell line and identification of kinetochore-containing (CREST staining) micronuclei in human lymphocytes",
abstract = "Quercetin, a mutagenic flavonoid widely distributed in edible plants, was studied for the induction of micronuclei (MN). We have carried out the MN assay in bone marrow polychromatic erythrocytes in mice, in cytokinesis-blocked human lymphocytes and in cytokinesis-blocked V79 cells. MN assay in vitro was performed in the presence and in the absence of S9. To further extend the study, an antikinetochore antibody (CREST staining) was used to distinguish MN containing whole chromosomes (kinetochore positive) from those containing acentric fragments (kinetochore negative). When tested in vivo quercetin failed to induce micronuclei, a result which is in agreement with other published reports. When tested in vitro in V79 cells quercetin clearly induces micronuclei in the absence of S9 and also in the presence of S9 for the highest dose used. When tested in vitro in human lymphocytes quercetin shows a significant induction of micronuclei in the absence and in the presence of S9. The presence of s9 compared to its absence is not significant for any of the systems used. Both in the presence and absence of S9, quercetin appears to behave as a clastogenic agent in human lymphocytes inducing a significant majority of kinetochore-negative MN.",
keywords = "Antikinetochore antibody, Human lymphocyte, Micronucleus, Mouse bone marrow, Quercetin, V79 cell line",
author = "Helena Caria and Teresa Chaveca and Ant{\'o}nio Laires and Jos{\'e} Rueff",
year = "1995",
month = "1",
day = "1",
doi = "10.1016/0165-1218(95)90075-6",
language = "English",
volume = "343",
pages = "85--94",
journal = "Mutation research-Genetic toxicology",
issn = "0165-1218",
publisher = "Elsevier Science B.V., Inc",
number = "2-3",

}

TY - JOUR

T1 - Genotoxicity of quercetin in the micronucleus assay in mouse bone marrow erythrocytes, human lymphocytes, V79 cell line and identification of kinetochore-containing (CREST staining) micronuclei in human lymphocytes

AU - Caria, Helena

AU - Chaveca, Teresa

AU - Laires, António

AU - Rueff, José

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Quercetin, a mutagenic flavonoid widely distributed in edible plants, was studied for the induction of micronuclei (MN). We have carried out the MN assay in bone marrow polychromatic erythrocytes in mice, in cytokinesis-blocked human lymphocytes and in cytokinesis-blocked V79 cells. MN assay in vitro was performed in the presence and in the absence of S9. To further extend the study, an antikinetochore antibody (CREST staining) was used to distinguish MN containing whole chromosomes (kinetochore positive) from those containing acentric fragments (kinetochore negative). When tested in vivo quercetin failed to induce micronuclei, a result which is in agreement with other published reports. When tested in vitro in V79 cells quercetin clearly induces micronuclei in the absence of S9 and also in the presence of S9 for the highest dose used. When tested in vitro in human lymphocytes quercetin shows a significant induction of micronuclei in the absence and in the presence of S9. The presence of s9 compared to its absence is not significant for any of the systems used. Both in the presence and absence of S9, quercetin appears to behave as a clastogenic agent in human lymphocytes inducing a significant majority of kinetochore-negative MN.

AB - Quercetin, a mutagenic flavonoid widely distributed in edible plants, was studied for the induction of micronuclei (MN). We have carried out the MN assay in bone marrow polychromatic erythrocytes in mice, in cytokinesis-blocked human lymphocytes and in cytokinesis-blocked V79 cells. MN assay in vitro was performed in the presence and in the absence of S9. To further extend the study, an antikinetochore antibody (CREST staining) was used to distinguish MN containing whole chromosomes (kinetochore positive) from those containing acentric fragments (kinetochore negative). When tested in vivo quercetin failed to induce micronuclei, a result which is in agreement with other published reports. When tested in vitro in V79 cells quercetin clearly induces micronuclei in the absence of S9 and also in the presence of S9 for the highest dose used. When tested in vitro in human lymphocytes quercetin shows a significant induction of micronuclei in the absence and in the presence of S9. The presence of s9 compared to its absence is not significant for any of the systems used. Both in the presence and absence of S9, quercetin appears to behave as a clastogenic agent in human lymphocytes inducing a significant majority of kinetochore-negative MN.

KW - Antikinetochore antibody

KW - Human lymphocyte

KW - Micronucleus

KW - Mouse bone marrow

KW - Quercetin

KW - V79 cell line

UR - http://www.scopus.com/inward/record.url?scp=0029077797&partnerID=8YFLogxK

U2 - 10.1016/0165-1218(95)90075-6

DO - 10.1016/0165-1218(95)90075-6

M3 - Article

VL - 343

SP - 85

EP - 94

JO - Mutation research-Genetic toxicology

JF - Mutation research-Genetic toxicology

SN - 0165-1218

IS - 2-3

ER -