Genotoxicity of instant coffee and of some phenolic compounds present in coffee upon nitrosation

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Instant coffee exhibits genotoxic activity upon nitrosation at acidic pH values in the Ames tester strain TA100. Using adsorption chromatography (Amberlit XAD-2) it was observed that the major fraction of molecules responsible for the genotoxic activity upon nitrosation was not retained on this resin, suggesting that the polar molecules present in instant coffee could be responsible for the genotoxicity observed upon nitrosation. Some phenolic molecules present in instant coffee (catechol, caffeic acid, and chlorogenic acid) were also genotoxic upon nitrosation under the same experimental conditions. The concentrations of nitrosatable phenolic compounds in the studied coffee were determined by HPLC and their contributions to the total genotoxicity observed were studied. The results obtained suggest that besides phenolic compounds other molecules were also involved in the genotoxicity of this beverage upon nitrosation. Teratogenesis Carcinog.
Original languageUnknown
Pages (from-to)241-249
JournalTeratogenesis Carcinogenesis And Mutagenesis
Volume20
Issue number4
DOIs
Publication statusPublished - 1 Jan 2000

Cite this

@article{ad174dd1ad4c4629b7182d73c9cb6131,
title = "Genotoxicity of instant coffee and of some phenolic compounds present in coffee upon nitrosation",
abstract = "Instant coffee exhibits genotoxic activity upon nitrosation at acidic pH values in the Ames tester strain TA100. Using adsorption chromatography (Amberlit XAD-2) it was observed that the major fraction of molecules responsible for the genotoxic activity upon nitrosation was not retained on this resin, suggesting that the polar molecules present in instant coffee could be responsible for the genotoxicity observed upon nitrosation. Some phenolic molecules present in instant coffee (catechol, caffeic acid, and chlorogenic acid) were also genotoxic upon nitrosation under the same experimental conditions. The concentrations of nitrosatable phenolic compounds in the studied coffee were determined by HPLC and their contributions to the total genotoxicity observed were studied. The results obtained suggest that besides phenolic compounds other molecules were also involved in the genotoxicity of this beverage upon nitrosation. Teratogenesis Carcinog.",
keywords = "mutagenicity, NITRITE, SALTED FISH, PRECURSORS, polyphenols, coffee, nitrosation, TYRAMINE, PRODUCTS, MEAT, MUTAGENICITY, BEANS",
author = "MP Duarte and A Laires and JF Gaspar and J. Rueff",
note = "WOS:000088494500006",
year = "2000",
month = "1",
day = "1",
doi = "10.1002/1520-6866(2000)20:4241::AID-TCM6>3.0.CO;2-4",
language = "Unknown",
volume = "20",
pages = "241--249",
journal = "Teratogenesis Carcinogenesis And Mutagenesis",
issn = "0270-3211",
publisher = "Wiley",
number = "4",

}

TY - JOUR

T1 - Genotoxicity of instant coffee and of some phenolic compounds present in coffee upon nitrosation

AU - Duarte, MP

AU - Laires, A

AU - Gaspar, JF

AU - Rueff, J.

N1 - WOS:000088494500006

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Instant coffee exhibits genotoxic activity upon nitrosation at acidic pH values in the Ames tester strain TA100. Using adsorption chromatography (Amberlit XAD-2) it was observed that the major fraction of molecules responsible for the genotoxic activity upon nitrosation was not retained on this resin, suggesting that the polar molecules present in instant coffee could be responsible for the genotoxicity observed upon nitrosation. Some phenolic molecules present in instant coffee (catechol, caffeic acid, and chlorogenic acid) were also genotoxic upon nitrosation under the same experimental conditions. The concentrations of nitrosatable phenolic compounds in the studied coffee were determined by HPLC and their contributions to the total genotoxicity observed were studied. The results obtained suggest that besides phenolic compounds other molecules were also involved in the genotoxicity of this beverage upon nitrosation. Teratogenesis Carcinog.

AB - Instant coffee exhibits genotoxic activity upon nitrosation at acidic pH values in the Ames tester strain TA100. Using adsorption chromatography (Amberlit XAD-2) it was observed that the major fraction of molecules responsible for the genotoxic activity upon nitrosation was not retained on this resin, suggesting that the polar molecules present in instant coffee could be responsible for the genotoxicity observed upon nitrosation. Some phenolic molecules present in instant coffee (catechol, caffeic acid, and chlorogenic acid) were also genotoxic upon nitrosation under the same experimental conditions. The concentrations of nitrosatable phenolic compounds in the studied coffee were determined by HPLC and their contributions to the total genotoxicity observed were studied. The results obtained suggest that besides phenolic compounds other molecules were also involved in the genotoxicity of this beverage upon nitrosation. Teratogenesis Carcinog.

KW - mutagenicity

KW - NITRITE

KW - SALTED FISH

KW - PRECURSORS

KW - polyphenols

KW - coffee

KW - nitrosation

KW - TYRAMINE

KW - PRODUCTS

KW - MEAT

KW - MUTAGENICITY

KW - BEANS

U2 - 10.1002/1520-6866(2000)20:4241::AID-TCM6>3.0.CO;2-4

DO - 10.1002/1520-6866(2000)20:4241::AID-TCM6>3.0.CO;2-4

M3 - Article

VL - 20

SP - 241

EP - 249

JO - Teratogenesis Carcinogenesis And Mutagenesis

JF - Teratogenesis Carcinogenesis And Mutagenesis

SN - 0270-3211

IS - 4

ER -