Genotoxicity assessment of aromatic-amines and amides in genetically-engineered v79 cells

ID Silva, M. H. Caria, A. Laires, T Chaveca, HR Glatt, J. Rueff, AS Rodrigues

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A genetically engineered V79 cell line expressing rat CYP1A2 and another cell line expressing rat CYP1A2 as well as endogenous acetyltransferase activity, as well as CYP-deficient parental V79 cell lines, were used to assess the genotoxicity of the aromatic amines and amides 2-aminoanthracene, 2-aminofluorene, 2-acetylaminofluorene, 4-acetylaminofluorene and 2-amino-3-methylimidazo[4,5-f]quinoline, with chromosomal aberrations and sister chromatid exchanges as the end-points. None of the test compounds showed a clear effect on the frequency of chromosomal aberrations in any cell line used. Sister chromatid exchanges, however, were induced by 2-aminoanthracene, 2-aminofluorene and 2-acetylaminofluorene in the CYP1A2-proficient cells, but not in the CYP1A2-deficient cells. The presence of acetyltransferase activity enhanced the effect of 2-aminoanthracene, 2-aminofluorene and 2-acetylaminofluorene. 4-Acetylaminofluorene and 2-amino-3-methylimidazo[4,5-f]quinoline did not induce sister chromatid exchanges in the investigated cell lines. The use of cell lines with defined metabolic capabilities seems to be a valuable tool to study specific metabolic pathways important in the activation of procarcinogens.

Original languageEnglish
Pages (from-to)93-100
Number of pages8
JournalMutation research-Genetic toxicology
Volume341
Issue number2
DOIs
Publication statusPublished - Dec 1994

Keywords

  • GENETICALLY ENGINEERED V79 CELL
  • CYTOCHROME P450
  • CHROMOSOMAL ABERRATIONS
  • SISTER CHROMATID EXCHANGE
  • METABOLIC ACTIVATION
  • AROMATIC AMINE AND AMIDE
  • CHINESE-HAMSTER-CELLS
  • SISTER-CHROMATID EXCHANGES
  • STABLE EXPRESSION
  • BACTERIAL MUTAGENICITY
  • METABOLIC-ACTIVATION
  • CDNA
  • CARCINOGENS
  • TOXICOLOGY
  • INVITRO
  • PROMUTAGENS

Cite this

@article{93048130dcc94e08b733189bdfab0ecf,
title = "Genotoxicity assessment of aromatic-amines and amides in genetically-engineered v79 cells",
abstract = "A genetically engineered V79 cell line expressing rat CYP1A2 and another cell line expressing rat CYP1A2 as well as endogenous acetyltransferase activity, as well as CYP-deficient parental V79 cell lines, were used to assess the genotoxicity of the aromatic amines and amides 2-aminoanthracene, 2-aminofluorene, 2-acetylaminofluorene, 4-acetylaminofluorene and 2-amino-3-methylimidazo[4,5-f]quinoline, with chromosomal aberrations and sister chromatid exchanges as the end-points. None of the test compounds showed a clear effect on the frequency of chromosomal aberrations in any cell line used. Sister chromatid exchanges, however, were induced by 2-aminoanthracene, 2-aminofluorene and 2-acetylaminofluorene in the CYP1A2-proficient cells, but not in the CYP1A2-deficient cells. The presence of acetyltransferase activity enhanced the effect of 2-aminoanthracene, 2-aminofluorene and 2-acetylaminofluorene. 4-Acetylaminofluorene and 2-amino-3-methylimidazo[4,5-f]quinoline did not induce sister chromatid exchanges in the investigated cell lines. The use of cell lines with defined metabolic capabilities seems to be a valuable tool to study specific metabolic pathways important in the activation of procarcinogens.",
keywords = "GENETICALLY ENGINEERED V79 CELL, CYTOCHROME P450, CHROMOSOMAL ABERRATIONS, SISTER CHROMATID EXCHANGE, METABOLIC ACTIVATION, AROMATIC AMINE AND AMIDE, CHINESE-HAMSTER-CELLS, SISTER-CHROMATID EXCHANGES, STABLE EXPRESSION, BACTERIAL MUTAGENICITY, METABOLIC-ACTIVATION, CDNA, CARCINOGENS, TOXICOLOGY, INVITRO, PROMUTAGENS",
author = "ID Silva and Caria, {M. H.} and A. Laires and T Chaveca and HR Glatt and J. Rueff and AS Rodrigues",
year = "1994",
month = "12",
doi = "10.1016/0165-1218(94)90091-4",
language = "English",
volume = "341",
pages = "93--100",
journal = "Mutation research-Genetic toxicology",
issn = "0165-1218",
publisher = "Elsevier Science B.V., Inc",
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}

Genotoxicity assessment of aromatic-amines and amides in genetically-engineered v79 cells. / Silva, ID; Caria, M. H.; Laires, A.; Chaveca, T; Glatt, HR; Rueff, J.; Rodrigues, AS.

In: Mutation research-Genetic toxicology, Vol. 341, No. 2, 12.1994, p. 93-100.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Genotoxicity assessment of aromatic-amines and amides in genetically-engineered v79 cells

AU - Silva, ID

AU - Caria, M. H.

AU - Laires, A.

AU - Chaveca, T

AU - Glatt, HR

AU - Rueff, J.

AU - Rodrigues, AS

PY - 1994/12

Y1 - 1994/12

N2 - A genetically engineered V79 cell line expressing rat CYP1A2 and another cell line expressing rat CYP1A2 as well as endogenous acetyltransferase activity, as well as CYP-deficient parental V79 cell lines, were used to assess the genotoxicity of the aromatic amines and amides 2-aminoanthracene, 2-aminofluorene, 2-acetylaminofluorene, 4-acetylaminofluorene and 2-amino-3-methylimidazo[4,5-f]quinoline, with chromosomal aberrations and sister chromatid exchanges as the end-points. None of the test compounds showed a clear effect on the frequency of chromosomal aberrations in any cell line used. Sister chromatid exchanges, however, were induced by 2-aminoanthracene, 2-aminofluorene and 2-acetylaminofluorene in the CYP1A2-proficient cells, but not in the CYP1A2-deficient cells. The presence of acetyltransferase activity enhanced the effect of 2-aminoanthracene, 2-aminofluorene and 2-acetylaminofluorene. 4-Acetylaminofluorene and 2-amino-3-methylimidazo[4,5-f]quinoline did not induce sister chromatid exchanges in the investigated cell lines. The use of cell lines with defined metabolic capabilities seems to be a valuable tool to study specific metabolic pathways important in the activation of procarcinogens.

AB - A genetically engineered V79 cell line expressing rat CYP1A2 and another cell line expressing rat CYP1A2 as well as endogenous acetyltransferase activity, as well as CYP-deficient parental V79 cell lines, were used to assess the genotoxicity of the aromatic amines and amides 2-aminoanthracene, 2-aminofluorene, 2-acetylaminofluorene, 4-acetylaminofluorene and 2-amino-3-methylimidazo[4,5-f]quinoline, with chromosomal aberrations and sister chromatid exchanges as the end-points. None of the test compounds showed a clear effect on the frequency of chromosomal aberrations in any cell line used. Sister chromatid exchanges, however, were induced by 2-aminoanthracene, 2-aminofluorene and 2-acetylaminofluorene in the CYP1A2-proficient cells, but not in the CYP1A2-deficient cells. The presence of acetyltransferase activity enhanced the effect of 2-aminoanthracene, 2-aminofluorene and 2-acetylaminofluorene. 4-Acetylaminofluorene and 2-amino-3-methylimidazo[4,5-f]quinoline did not induce sister chromatid exchanges in the investigated cell lines. The use of cell lines with defined metabolic capabilities seems to be a valuable tool to study specific metabolic pathways important in the activation of procarcinogens.

KW - GENETICALLY ENGINEERED V79 CELL

KW - CYTOCHROME P450

KW - CHROMOSOMAL ABERRATIONS

KW - SISTER CHROMATID EXCHANGE

KW - METABOLIC ACTIVATION

KW - AROMATIC AMINE AND AMIDE

KW - CHINESE-HAMSTER-CELLS

KW - SISTER-CHROMATID EXCHANGES

KW - STABLE EXPRESSION

KW - BACTERIAL MUTAGENICITY

KW - METABOLIC-ACTIVATION

KW - CDNA

KW - CARCINOGENS

KW - TOXICOLOGY

KW - INVITRO

KW - PROMUTAGENS

U2 - 10.1016/0165-1218(94)90091-4

DO - 10.1016/0165-1218(94)90091-4

M3 - Article

VL - 341

SP - 93

EP - 100

JO - Mutation research-Genetic toxicology

JF - Mutation research-Genetic toxicology

SN - 0165-1218

IS - 2

ER -