Genotoxicity assessment of aromatic-amines and amides in genetically-engineered v79 cells

ID Silva, M. H. Caria, A. Laires, T Chaveca, HR Glatt, J. Rueff, AS Rodrigues

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18 Citations (Scopus)

Abstract

A genetically engineered V79 cell line expressing rat CYP1A2 and another cell line expressing rat CYP1A2 as well as endogenous acetyltransferase activity, as well as CYP-deficient parental V79 cell lines, were used to assess the genotoxicity of the aromatic amines and amides 2-aminoanthracene, 2-aminofluorene, 2-acetylaminofluorene, 4-acetylaminofluorene and 2-amino-3-methylimidazo[4,5-f]quinoline, with chromosomal aberrations and sister chromatid exchanges as the end-points. None of the test compounds showed a clear effect on the frequency of chromosomal aberrations in any cell line used. Sister chromatid exchanges, however, were induced by 2-aminoanthracene, 2-aminofluorene and 2-acetylaminofluorene in the CYP1A2-proficient cells, but not in the CYP1A2-deficient cells. The presence of acetyltransferase activity enhanced the effect of 2-aminoanthracene, 2-aminofluorene and 2-acetylaminofluorene. 4-Acetylaminofluorene and 2-amino-3-methylimidazo[4,5-f]quinoline did not induce sister chromatid exchanges in the investigated cell lines. The use of cell lines with defined metabolic capabilities seems to be a valuable tool to study specific metabolic pathways important in the activation of procarcinogens.

Original languageEnglish
Pages (from-to)93-100
Number of pages8
JournalMutation research-Genetic toxicology
Volume341
Issue number2
DOIs
Publication statusPublished - Dec 1994

Keywords

  • GENETICALLY ENGINEERED V79 CELL
  • CYTOCHROME P450
  • CHROMOSOMAL ABERRATIONS
  • SISTER CHROMATID EXCHANGE
  • METABOLIC ACTIVATION
  • AROMATIC AMINE AND AMIDE
  • CHINESE-HAMSTER-CELLS
  • SISTER-CHROMATID EXCHANGES
  • STABLE EXPRESSION
  • BACTERIAL MUTAGENICITY
  • METABOLIC-ACTIVATION
  • CDNA
  • CARCINOGENS
  • TOXICOLOGY
  • INVITRO
  • PROMUTAGENS

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