Genomic insights into specialized metabolism in the marine actinomycete Salinispora

Anne Catrin Letzel, Jing Li, Gregory C.A. Amos, Natalie Millán-Aguiñaga, Joape Ginigini, Usama R. Abdelmohsen, Susana P. Gaudêncio, Nadine Ziemert, Bradley S. Moore, Paul R. Jensen

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Comparative genomics is providing new opportunities to address the diversity and distributions of genes encoding the biosynthesis of specialized metabolites. An analysis of 119 genome sequences representing three closely related species of the marine actinomycete genus Salinispora reveals extraordinary biosynthetic diversity in the form of 176 distinct biosynthetic gene clusters (BGCs) of which only 24 have been linked to their products. Remarkably, more than half of the BGCs were observed in only one or two strains, suggesting they were acquired relatively recently in the evolutionary history of the genus. These acquired gene clusters are concentrated in specific genomic islands, which represent hot spots for BGC acquisition. While most BGCs are stable in terms of their chromosomal position, others migrated to different locations or were exchanged with unrelated gene clusters suggesting a plug and play type model of evolution that provides a mechanism to test the relative fitness effects of specialized metabolites. Transcriptome analyses were used to address the relationships between BGC abundance, chromosomal position and product discovery. The results indicate that recently acquired BGCs can be functional and that complex evolutionary processes shape the micro-diversity of specialized metabolism observed in closely related environmental bacteria.

Original languageEnglish
Pages (from-to)3660-3673
Number of pages14
JournalEnvironmental Microbiology
Volume19
Issue number9
DOIs
Publication statusPublished - 1 Sep 2017

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Actinobacteria
Multigene Family
multigene family
genomics
metabolism
gene
metabolite
genomic islands
metabolites
Genomic Islands
Gene Expression Profiling
Genomics
transcriptome
History
biosynthesis
Genome
fitness
genome
Bacteria
history

Cite this

Letzel, A. C., Li, J., Amos, G. C. A., Millán-Aguiñaga, N., Ginigini, J., Abdelmohsen, U. R., ... Jensen, P. R. (2017). Genomic insights into specialized metabolism in the marine actinomycete Salinispora. Environmental Microbiology, 19(9), 3660-3673. https://doi.org/10.1111/1462-2920.13867
Letzel, Anne Catrin ; Li, Jing ; Amos, Gregory C.A. ; Millán-Aguiñaga, Natalie ; Ginigini, Joape ; Abdelmohsen, Usama R. ; Gaudêncio, Susana P. ; Ziemert, Nadine ; Moore, Bradley S. ; Jensen, Paul R. / Genomic insights into specialized metabolism in the marine actinomycete Salinispora. In: Environmental Microbiology. 2017 ; Vol. 19, No. 9. pp. 3660-3673.
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Letzel, AC, Li, J, Amos, GCA, Millán-Aguiñaga, N, Ginigini, J, Abdelmohsen, UR, Gaudêncio, SP, Ziemert, N, Moore, BS & Jensen, PR 2017, 'Genomic insights into specialized metabolism in the marine actinomycete Salinispora', Environmental Microbiology, vol. 19, no. 9, pp. 3660-3673. https://doi.org/10.1111/1462-2920.13867

Genomic insights into specialized metabolism in the marine actinomycete Salinispora. / Letzel, Anne Catrin; Li, Jing; Amos, Gregory C.A.; Millán-Aguiñaga, Natalie; Ginigini, Joape; Abdelmohsen, Usama R.; Gaudêncio, Susana P.; Ziemert, Nadine; Moore, Bradley S.; Jensen, Paul R.

In: Environmental Microbiology, Vol. 19, No. 9, 01.09.2017, p. 3660-3673.

Research output: Contribution to journalArticle

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AU - Jensen, Paul R.

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Letzel AC, Li J, Amos GCA, Millán-Aguiñaga N, Ginigini J, Abdelmohsen UR et al. Genomic insights into specialized metabolism in the marine actinomycete Salinispora. Environmental Microbiology. 2017 Sep 1;19(9):3660-3673. https://doi.org/10.1111/1462-2920.13867