Generation and Characterization of Monoclonal Antibodies Against Rift Valley Fever Virus Nucleoprotein

J. M. Fafetine, A. Domingos, S. Antunes, A. Esteves, J. T. Paweska, J. A.W. Coetzer, V. P.M.G. Rutten, L. Neves

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Summary: Due to the unpredictable and explosive nature of Rift Valley fever (RVF) outbreaks, rapid and accurate diagnostic assays for low-resource settings are urgently needed. To improve existing diagnostic assays, monoclonal antibodies (MAbs) specific for the nucleocapsid protein of RVF virus (RVFV) were produced and characterized. Four IgG2a MAbs showed specific binding to denatured nucleocapsid protein, both from a recombinant source and from inactivated RVFV, in Western blot analysis and in an enzyme-linked immunosorbent assay (ELISA). Cross-reactivity with genetically related and non-related arboviruses including Bunyamwera and Calovo viruses (Bunyaviridae family), West Nile and Dengue-2 viruses (Flaviviridae family), and Sindbis and Chikungunya viruses (Togaviridae family) was not detected. These MAbs represent a useful tool for the development of rapid diagnostic assays for early recognition of RVF.

Original languageEnglish
Pages (from-to)24-30
Number of pages7
JournalTransboundary and Emerging Diseases
Volume60
Issue numberSUPPL.2
DOIs
Publication statusPublished - 1 Nov 2013

Fingerprint

Rift Valley fever virus
nucleoproteins
Nucleoproteins
Bunyamwera virus
Rift Valley Fever
Rift Valley fever
nucleocapsid proteins
Nucleocapsid Proteins
monoclonal antibodies
Monoclonal Antibodies
assays
Togaviridae
Flaviviridae
Bunyaviridae
Chikungunya virus
Sindbis virus
Sindbis Virus
Arboviruses
Dengue virus
viruses

Keywords

  • Cross-reactivity
  • Monoclonal antibodies
  • Nucleocapsid protein
  • Rift Valley fever virus

Cite this

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abstract = "Summary: Due to the unpredictable and explosive nature of Rift Valley fever (RVF) outbreaks, rapid and accurate diagnostic assays for low-resource settings are urgently needed. To improve existing diagnostic assays, monoclonal antibodies (MAbs) specific for the nucleocapsid protein of RVF virus (RVFV) were produced and characterized. Four IgG2a MAbs showed specific binding to denatured nucleocapsid protein, both from a recombinant source and from inactivated RVFV, in Western blot analysis and in an enzyme-linked immunosorbent assay (ELISA). Cross-reactivity with genetically related and non-related arboviruses including Bunyamwera and Calovo viruses (Bunyaviridae family), West Nile and Dengue-2 viruses (Flaviviridae family), and Sindbis and Chikungunya viruses (Togaviridae family) was not detected. These MAbs represent a useful tool for the development of rapid diagnostic assays for early recognition of RVF.",
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Generation and Characterization of Monoclonal Antibodies Against Rift Valley Fever Virus Nucleoprotein. / Fafetine, J. M.; Domingos, A.; Antunes, S.; Esteves, A.; Paweska, J. T.; Coetzer, J. A.W.; Rutten, V. P.M.G.; Neves, L.

In: Transboundary and Emerging Diseases, Vol. 60, No. SUPPL.2, 01.11.2013, p. 24-30.

Research output: Contribution to journalArticle

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AU - Domingos, A.

AU - Antunes, S.

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