Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration

Selin Pars, Fernando Cristo, José M Inácio, Graça Rosas, Isabel Marques Carreira, Joana Barbosa Melo, Patrícia Mendes, Duarte Saraiva Martins, Luís Pereira de Almeida, José Maio, Rui Anjos, José A Belo

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Abstract

A DAND5-control human iPSC line was generated from the urinary cells of a phenotypically normal donor. Exfoliated renal epithelial (RE) cells were collected and reprogrammed into iPSCs using Sendai virus reprogramming system. The pluripotency, in vitro differentiation potential, karyotype stability, and the transgene-free status of generated iPSC line were analyzed and confirmed. This cell line can be exploited as a control iPSC line to better understand the mechanisms involved in DAND5-associated cardiac disease.

Original languageEnglish
Pages (from-to)202-206
Number of pages5
JournalStem Cell Research
Volume29
Early online date28 Apr 2018
DOIs
Publication statusPublished - May 2018

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Sendai virus
Transgenes
Karyotype
Heart Diseases
Epithelial Cells
Cells
Tissue Donors
Kidney
Cell Line
Viruses
In Vitro Techniques

Cite this

Pars, Selin ; Cristo, Fernando ; Inácio, José M ; Rosas, Graça ; Carreira, Isabel Marques ; Melo, Joana Barbosa ; Mendes, Patrícia ; Martins, Duarte Saraiva ; de Almeida, Luís Pereira ; Maio, José ; Anjos, Rui ; Belo, José A. / Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration. In: Stem Cell Research. 2018 ; Vol. 29. pp. 202-206.
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abstract = "A DAND5-control human iPSC line was generated from the urinary cells of a phenotypically normal donor. Exfoliated renal epithelial (RE) cells were collected and reprogrammed into iPSCs using Sendai virus reprogramming system. The pluripotency, in vitro differentiation potential, karyotype stability, and the transgene-free status of generated iPSC line were analyzed and confirmed. This cell line can be exploited as a control iPSC line to better understand the mechanisms involved in DAND5-associated cardiac disease.",
author = "Selin Pars and Fernando Cristo and In{\'a}cio, {Jos{\'e} M} and Gra{\cc}a Rosas and Carreira, {Isabel Marques} and Melo, {Joana Barbosa} and Patr{\'i}cia Mendes and Martins, {Duarte Saraiva} and {de Almeida}, {Lu{\'i}s Pereira} and Jos{\'e} Maio and Rui Anjos and Belo, {Jos{\'e} A}",
note = "info:eu-repo/grantAgreement/FCT/SFRH/SFRH{\%}2FBD{\%}2F81431{\%}2F2011/PT# info:eu-repo/grantAgreement/FCT/5876/147260/PT# We would like to thank the patient and their guardians for their generous donation of the urine sample used in this study. We also would like to thank Ana Jardim for technical support in karyotype analysis. This work was supported by Fundacao para a Ciencia e a Tecnologia (PTDC/BIM-MED/3363/2014). iNOVA4Health - UID/Multi/04462/2013, a program financially supported by Fundacao para a Ciencia e Tecnologia/Ministerio da Educacao e Ciencia, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is acknowledged.",
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Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration. / Pars, Selin; Cristo, Fernando; Inácio, José M; Rosas, Graça; Carreira, Isabel Marques; Melo, Joana Barbosa; Mendes, Patrícia; Martins, Duarte Saraiva; de Almeida, Luís Pereira; Maio, José; Anjos, Rui; Belo, José A.

In: Stem Cell Research, Vol. 29, 05.2018, p. 202-206.

Research output: Contribution to journalArticle

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