Gamma Irradiation Processing on 3D PCL Devices—A Preliminary Biocompatibility Assessment

Fernando Guedes, Mariana V. Branquinho, Sara Biscaia, Rui D. Alvites, Ana C. Sousa, Bruna Lopes, Patrícia Sousa, Alexandra Rêma, Irina Amorim, Fátima Faria, Tatiana M. Patrício, Nuno Alves, António Bugalho, Ana C. Maurício

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2 Citations (Scopus)
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Additive manufacturing or 3D printing applying polycaprolactone (PCL)-based medical devices represents an important branch of tissue engineering, where the sterilization method is a key process for further safe application in vitro and in vivo. In this study, the authors intend to access the most suitable gamma radiation conditions to sterilize PCL-based scaffolds in a preliminary biocompatibility assessment, envisioning future studies for airway obstruction conditions. Three radiation levels were considered, 25 kGy, 35 kGy and 45 kGy, and evaluated as regards their cyto- and biocompatibility. All three groups presented biocompatible properties, indicating an adequate sterility condition. As for the cytocompatibility analysis, devices sterilized with 35 kGy and 45 kGy showed better results, with the 45 kGy showing overall improved outcomes. This study allowed the selection of the most suitable sterilization condition for PCL-based scaffolds, aiming at immediate future assays, by applying 3D-customized printing techniques to specific airway obstruction lesions of the trachea.

Original languageEnglish
Article number15916
JournalInternational Journal of Molecular Sciences
Issue number24
Publication statusPublished - Dec 2022


  • 3D printing
  • biocompatibility
  • cytocompatibility
  • gamma irradiation
  • mesenchymal stem cells
  • polycaprolactone


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