Fungal biodeterioration of stained-glass windows in monuments from Belém do Pará (Brazil)

A. M. Corrêa Pinto, T. Palomar, L. C. Alves, S. H. M. da Silva, R. C. Monteiro, M. F. Macedo, M. G. Vilarigues

Research output: Contribution to journalArticle

Abstract

The most prominent historical buildings in Belém do Pará (Northern Brazil) have modernist stained-glass windows, which were commissioned from Europe since the end of the 19th century. Some of them present biodegradation; however, there is no information about the microbial activity on them. The present work is focused on the biodeterioration by fungi on some of these Modern stained-glass windows. The fungal communities were collected, isolated and then identified by means of molecular methods. Additionally, a laboratory-based biodeterioration experiment was carried out to assess the fungal activity on replica glass samples with three different chemical compositions. The replica samples were inoculated with a four-fungal species mixture and incubated under optimal growth conditions for 5 months. Optical microscopy, μ-PIXE, SEM-EDS and FTIR-ATR were performed to evaluate the biodeterioration of the soda-lime silicate glasses. This multidisciplinary approach showed that the inoculated spores (Aspergillus arenarioides, Fusarium oxysporum, Hortaea werneckii, and Trichoderma longibrachiatum) were able to form substantial mycelia in all replica glass samples. The main alterations observed were small crystals, hyphae fingerprints and a slight decrease on the glass surface smoothness. Despite the aforementioned damages, the soda-lime silicate glass compositions showed high resistance against the inoculated fungal species.

Original languageEnglish
Pages (from-to)106-113
Number of pages8
JournalInternational Biodeterioration and Biodegradation
Volume138
DOIs
Publication statusPublished - 1 Mar 2019

Fingerprint

Stained glass
monument
Glass
Brazil
biodegradation
glass
Silicates
Lime
lime
Aspergillus
silicate
Biodegradation
Fungi
Chemical analysis
Optical microscopy
Trichoderma
Energy dispersive spectroscopy
Hyphae
Mycelium
Fusarium

Keywords

  • Biodeterioration
  • Fungal identification
  • Stained-glass windows
  • Tropical climate

Cite this

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title = "Fungal biodeterioration of stained-glass windows in monuments from Bel{\'e}m do Par{\'a} (Brazil)",
abstract = "The most prominent historical buildings in Bel{\'e}m do Par{\'a} (Northern Brazil) have modernist stained-glass windows, which were commissioned from Europe since the end of the 19th century. Some of them present biodegradation; however, there is no information about the microbial activity on them. The present work is focused on the biodeterioration by fungi on some of these Modern stained-glass windows. The fungal communities were collected, isolated and then identified by means of molecular methods. Additionally, a laboratory-based biodeterioration experiment was carried out to assess the fungal activity on replica glass samples with three different chemical compositions. The replica samples were inoculated with a four-fungal species mixture and incubated under optimal growth conditions for 5 months. Optical microscopy, μ-PIXE, SEM-EDS and FTIR-ATR were performed to evaluate the biodeterioration of the soda-lime silicate glasses. This multidisciplinary approach showed that the inoculated spores (Aspergillus arenarioides, Fusarium oxysporum, Hortaea werneckii, and Trichoderma longibrachiatum) were able to form substantial mycelia in all replica glass samples. The main alterations observed were small crystals, hyphae fingerprints and a slight decrease on the glass surface smoothness. Despite the aforementioned damages, the soda-lime silicate glass compositions showed high resistance against the inoculated fungal species.",
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Fungal biodeterioration of stained-glass windows in monuments from Belém do Pará (Brazil). / Pinto, A. M. Corrêa ; Palomar, T.; Alves, L. C.; da Silva, S. H. M.; Monteiro, R. C.; Macedo, M. F.; Vilarigues, M. G.

In: International Biodeterioration and Biodegradation, Vol. 138, 01.03.2019, p. 106-113.

Research output: Contribution to journalArticle

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T1 - Fungal biodeterioration of stained-glass windows in monuments from Belém do Pará (Brazil)

AU - Pinto, A. M. Corrêa

AU - Palomar, T.

AU - Alves, L. C.

AU - da Silva, S. H. M.

AU - Monteiro, R. C.

AU - Macedo, M. F.

AU - Vilarigues, M. G.

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AB - The most prominent historical buildings in Belém do Pará (Northern Brazil) have modernist stained-glass windows, which were commissioned from Europe since the end of the 19th century. Some of them present biodegradation; however, there is no information about the microbial activity on them. The present work is focused on the biodeterioration by fungi on some of these Modern stained-glass windows. The fungal communities were collected, isolated and then identified by means of molecular methods. Additionally, a laboratory-based biodeterioration experiment was carried out to assess the fungal activity on replica glass samples with three different chemical compositions. The replica samples were inoculated with a four-fungal species mixture and incubated under optimal growth conditions for 5 months. Optical microscopy, μ-PIXE, SEM-EDS and FTIR-ATR were performed to evaluate the biodeterioration of the soda-lime silicate glasses. This multidisciplinary approach showed that the inoculated spores (Aspergillus arenarioides, Fusarium oxysporum, Hortaea werneckii, and Trichoderma longibrachiatum) were able to form substantial mycelia in all replica glass samples. The main alterations observed were small crystals, hyphae fingerprints and a slight decrease on the glass surface smoothness. Despite the aforementioned damages, the soda-lime silicate glass compositions showed high resistance against the inoculated fungal species.

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