TY - JOUR
T1 - Functional responses of Hyalella meinerti after exposure to environmentally realistic concentrations of 2,4-D, fipronil, and vinasse (individually and in mixture)
AU - Pinto, Thandy Junio da Silva
AU - Freitas, Juliane Silberschmidt
AU - Moreira, Raquel Aparecida
AU - Silva, Laís Conceição Menezes da
AU - Yoshii, Maria Paula Cardoso
AU - Lopes, Laís Fernanda de Palma
AU - Goulart, Bianca Veloso
AU - Vanderlei, Marina Reghini
AU - Athayde, Danillo Badolato
AU - Fraga, Priscille Dreux
AU - Ogura, Allan Pretti
AU - Schiesari, Luis
AU - Montagner, Cassiana Carolina
AU - Daam, Michiel Adriaan
AU - Espindola, Evaldo Luiz Gaeta
N1 - This work was supported by the Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Brazil, grant no. 2015/18790-3). T.J.S.P., L.C.M.S., M.P.C.Y., L.F.P.L., B.V.G., M.R.V., and A.P.O. have a Ph.D. scholarship from the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil) and Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brazil). J.S.F and R.A.M. have a pos doctoral grant from FAPESP (grant no. 2017/13377-6 and2017/241264, respectively). Financial support was also provided to M.A.D. by the Portuguese government (Fundacao para a Ciencia e Tecnologia (FCT, Brazil) through the research unit UIDB/04085/2020 (CENSE).
PY - 2021/2
Y1 - 2021/2
N2 - Sugarcane crops management in Brazil includes the use of pesticides, as well as alternative organic fertilizers such as vinasse obtained from waste of the ethanol industry. In order to assess the effects of the environmental contamination generated by such sugarcane practices, this study was aimed to investigate the effects of the pesticides 2,4-Dichlorophenoxyacetic acid (2,4-D) and fipronil, as well as vinasse, on the survival, behavior, and reproduction of the native epibenthic macroinvertebrate Hyalella meinerti through in situ and laboratory experiments. In situ assays were conducted in mesocosms with six treatments, i.e. untreated control, 2,4-D, fipronil, and vinasse, the mixture of the two pesticides, and both pesticides mixed with vinasse. Survival, swimming behavior, and reproduction were evaluated over time post contamination, from 0−96 h (T1) and 7–14 days (T2) through in situ experiments and 30–44 days (T3) and 75–89 days (T4) post contamination by laboratory bioassays with mesocosm water. In the T1 period, survival of H. meinerti was registered only in controls and mesocosms treated with 2,4-D. In the T2 period, treatments containing fipronil and vinasse (isolated or in both mixture treatments) still caused 100 % of mortality. Survival was recorded only in 2,4-D and control treatments, whereas reproduction only occurred in the control. In the T3 period, no survival occurred to fipronil and both mixture treatments. Vinasse and 2,4-D decreased total reproduction in comparison to control. In the T4 period, amphipods survival was detected when exposed to fipronil and its mixture with 2,4-D. However, these same treatments decreased the amplexus rates and total reproduction, with synergism denoted for the pesticide mixture. The swimming activity of males, females, and couples was decreased in surviving organisms exposed to 2,4-D, fipronil, vinasse, and the mixture of pesticides along all experimental periods. Our study showed that the application of fipronil, 2,4-D, and vinasse isolated or mixed at realistic concentrations of actual sugarcane management practices may negatively impact functional responses of indigenous amphipods in natural aquatic systems.
AB - Sugarcane crops management in Brazil includes the use of pesticides, as well as alternative organic fertilizers such as vinasse obtained from waste of the ethanol industry. In order to assess the effects of the environmental contamination generated by such sugarcane practices, this study was aimed to investigate the effects of the pesticides 2,4-Dichlorophenoxyacetic acid (2,4-D) and fipronil, as well as vinasse, on the survival, behavior, and reproduction of the native epibenthic macroinvertebrate Hyalella meinerti through in situ and laboratory experiments. In situ assays were conducted in mesocosms with six treatments, i.e. untreated control, 2,4-D, fipronil, and vinasse, the mixture of the two pesticides, and both pesticides mixed with vinasse. Survival, swimming behavior, and reproduction were evaluated over time post contamination, from 0−96 h (T1) and 7–14 days (T2) through in situ experiments and 30–44 days (T3) and 75–89 days (T4) post contamination by laboratory bioassays with mesocosm water. In the T1 period, survival of H. meinerti was registered only in controls and mesocosms treated with 2,4-D. In the T2 period, treatments containing fipronil and vinasse (isolated or in both mixture treatments) still caused 100 % of mortality. Survival was recorded only in 2,4-D and control treatments, whereas reproduction only occurred in the control. In the T3 period, no survival occurred to fipronil and both mixture treatments. Vinasse and 2,4-D decreased total reproduction in comparison to control. In the T4 period, amphipods survival was detected when exposed to fipronil and its mixture with 2,4-D. However, these same treatments decreased the amplexus rates and total reproduction, with synergism denoted for the pesticide mixture. The swimming activity of males, females, and couples was decreased in surviving organisms exposed to 2,4-D, fipronil, vinasse, and the mixture of pesticides along all experimental periods. Our study showed that the application of fipronil, 2,4-D, and vinasse isolated or mixed at realistic concentrations of actual sugarcane management practices may negatively impact functional responses of indigenous amphipods in natural aquatic systems.
KW - Behavior
KW - Mesocosms
KW - Pesticides
KW - Reproduction
KW - Synergism
UR - http://www.scopus.com/inward/record.url?scp=85097670730&partnerID=8YFLogxK
U2 - 10.1016/j.aquatox.2020.105712
DO - 10.1016/j.aquatox.2020.105712
M3 - Article
C2 - 33340833
AN - SCOPUS:85097670730
SN - 0166-445X
VL - 231
JO - Aquatic Toxicology
JF - Aquatic Toxicology
M1 - 105712
ER -