Functional properties of type I and type II cytochromes c3 from Desulfovibrio africanus

Catarina M. Paquete; Patrícia M. Pereira; Teresa Catarino; David L. Turner; Ricardo O. Louro; António V. Xavier

doi:10.1016/j.bbabio.2007.01.012

Functional properties of type I and type II cytochromes c₃ from Desulfovibrio africanus

Catarina M. Paquete, Patrícia M. Pereira, Teresa Catarino, David L. Turner, Ricardo O. Louro, António V. Xavier

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Type I cytochrome c₃ is a key protein in the bioenergetic metabolism of Desulfovibrio spp., mediating electron transfer between periplasmic hydrogenase and multihaem cytochromes associated with membrane bound complexes, such as type II cytochrome c₃. This work presents the NMR assignment of the haem substituents in type I cytochrome c₃ isolated from Desulfovibrio africanus and the thermodynamic and kinetic characterisation of type I and type II cytochromes c₃ belonging to the same organism. It is shown that the redox properties of the two proteins allow electrons to be transferred between them in the physiologically relevant direction with the release of energised protons close to the membrane where they can be used by the ATP synthase.

Original language	English
Pages (from-to)	178-188
Number of pages	11
Journal	Biochimica et Biophysica Acta-Bioenergetics
Volume	1767
Issue number	2
DOIs	https://doi.org/10.1016/j.bbabio.2007.01.012
Publication status	Published - Feb 2007

Keywords

Cytochrome c3
Desulfovibrio africanus
Electron transfer rates
Multicentre protein
Thermodynamic characterisation

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title = "Functional properties of type I and type II cytochromes c3 from Desulfovibrio africanus",

abstract = "Type I cytochrome c3 is a key protein in the bioenergetic metabolism of Desulfovibrio spp., mediating electron transfer between periplasmic hydrogenase and multihaem cytochromes associated with membrane bound complexes, such as type II cytochrome c3. This work presents the NMR assignment of the haem substituents in type I cytochrome c3 isolated from Desulfovibrio africanus and the thermodynamic and kinetic characterisation of type I and type II cytochromes c3 belonging to the same organism. It is shown that the redox properties of the two proteins allow electrons to be transferred between them in the physiologically relevant direction with the release of energised protons close to the membrane where they can be used by the ATP synthase.",

keywords = "Cytochrome c3, Desulfovibrio africanus, Electron transfer rates, Multicentre protein, Thermodynamic characterisation",

author = "Paquete, {Catarina M.} and Pereira, {Patr{\'i}cia M.} and Teresa Catarino and Turner, {David L.} and Louro, {Ricardo O.} and Xavier, {Ant{\'o}nio V.}",

note = "Funding Information: We would like to thank Jo{\~a}o Carita for the growth of the organisms and Isabel Pacheco for helping in the purification of the protein. Financial support was provided by contracts POCTI/QUI/47866/2002 and POCI/QUI/55690/2004 with Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia and fellowships to C. M. P. (SFRH/BD/6495/2001), to P.M.P (SFRH/BD/5231/2001) and to D.L.T. (SFRH/BCC/15305/2004) by Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2007",

month = feb,

doi = "10.1016/j.bbabio.2007.01.012",

language = "English",

volume = "1767",

pages = "178--188",

journal = "Biochimica et Biophysica Acta-Bioenergetics",

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AU - Paquete, Catarina M.

AU - Pereira, Patrícia M.

AU - Catarino, Teresa

AU - Turner, David L.

AU - Louro, Ricardo O.

AU - Xavier, António V.

N1 - Funding Information: We would like to thank João Carita for the growth of the organisms and Isabel Pacheco for helping in the purification of the protein. Financial support was provided by contracts POCTI/QUI/47866/2002 and POCI/QUI/55690/2004 with Fundação para a Ciência e Tecnologia and fellowships to C. M. P. (SFRH/BD/6495/2001), to P.M.P (SFRH/BD/5231/2001) and to D.L.T. (SFRH/BCC/15305/2004) by Fundação para a Ciência e Tecnologia. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2007/2

Y1 - 2007/2

N2 - Type I cytochrome c3 is a key protein in the bioenergetic metabolism of Desulfovibrio spp., mediating electron transfer between periplasmic hydrogenase and multihaem cytochromes associated with membrane bound complexes, such as type II cytochrome c3. This work presents the NMR assignment of the haem substituents in type I cytochrome c3 isolated from Desulfovibrio africanus and the thermodynamic and kinetic characterisation of type I and type II cytochromes c3 belonging to the same organism. It is shown that the redox properties of the two proteins allow electrons to be transferred between them in the physiologically relevant direction with the release of energised protons close to the membrane where they can be used by the ATP synthase.

AB - Type I cytochrome c3 is a key protein in the bioenergetic metabolism of Desulfovibrio spp., mediating electron transfer between periplasmic hydrogenase and multihaem cytochromes associated with membrane bound complexes, such as type II cytochrome c3. This work presents the NMR assignment of the haem substituents in type I cytochrome c3 isolated from Desulfovibrio africanus and the thermodynamic and kinetic characterisation of type I and type II cytochromes c3 belonging to the same organism. It is shown that the redox properties of the two proteins allow electrons to be transferred between them in the physiologically relevant direction with the release of energised protons close to the membrane where they can be used by the ATP synthase.

KW - Cytochrome c3

KW - Desulfovibrio africanus

KW - Electron transfer rates

KW - Multicentre protein

KW - Thermodynamic characterisation

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JO - Biochimica et Biophysica Acta-Bioenergetics

JF - Biochimica et Biophysica Acta-Bioenergetics

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ER -

Functional properties of type I and type II cytochromes c₃ from Desulfovibrio africanus

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