Functional characterization of PccH, a key cytochrome for electron transfer from electrodes to the bacterium Geobacter sulfurreducens

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The cytochrome PccH from Geobacter sulfurreducens (Gs) plays a crucial role in current-consuming fumarate-reducing biofilms. Deletion of pccH gene inhibited completely electron transfer from electrodes toward Gs cells. The pccH gene was cloned and the protein heterologously expressed in Escherichia coli. Complementary biophysical techniques including CD, UV-visible and NMR spectroscopy were used to characterize PccH. This cytochrome contains one low-spin c-type heme with His-Met axial coordination and unusual low-reduction potential. This reduction potential is pH-dependent, within the Gs physiological pH range, and is discussed within the context of the electron transfer mechanisms from electrodes to Gs cells.

Original languageEnglish
Pages (from-to)2662-2668
Number of pages7
JournalFEBS Letters
Volume587
Issue number16
DOIs
Publication statusPublished - 19 Aug 2013

Keywords

  • c-Type cytochrome
  • Electron transfer
  • Geobacter
  • Heme protein

Fingerprint Dive into the research topics of 'Functional characterization of PccH, a key cytochrome for electron transfer from electrodes to the bacterium Geobacter sulfurreducens'. Together they form a unique fingerprint.

  • Cite this