Functional and Physiological Methods of Evaluating Median Nerve Regeneration in the Rat

Diogo Casal, Eduarda Mota-Silva, Ines Iria, Diogo Pais, Ana Farinho, Sara Alves, Claudia Pen, Luis Mascarenhas-Lemos, Jose Ferreira-Silva, Mario Ferraz-Oliveira, Valentina Vassilenko, Paula A. Videira, Joao Goyri-O'Neill

Research output: Contribution to journalArticle

Abstract

The main goal of this investigation is to show how to create and repair different types of median nerve (MN) lesions in the rat. Moreover, different methods of simulating postoperative physiotherapy are presented. Multiple standardized strategies are used to assess motor and sensory recovery using an MN model of peripheral nerve lesion and repair, thus permitting easy comparison of the results. Several options are included for providing a postoperative physiotherapy-like environment to rats that have undergone MN injuries. Finally, the paper provides a method to evaluate the recovery of the MN using several noninvasive tests (i.e., grasping test, pin prick test, ladder rung walking test, rope climbing test, and walking track analysis), and physiological measurements (infrared thermography, electroneuromyography, flexion strength evaluation, and flexor carpi radialis muscle weight determination). Hence, this model seems particularly appropriate to replicate a clinical scenario, facilitating extrapolation of results to the human species. Although the sciatic nerve is the most studied nerve in peripheral nerve research, analysis of the rat MN presents various advantages. For example, there is a reduced incidence of joint contractures and automutilation of the affected limb in MN lesion studies. Furthermore, the MN is not covered by muscle masses, making its dissection easier than that of the sciatic nerve. In addition, MN recovery is observed sooner, because the MN is shorter than the sciatic nerve. Also, the MN has a parallel path to the ulnar nerve in the arm. Hence, the ulnar nerve can be easily used as the nerve graft for repairing MN injuries. Finally, the MN in rats is located in the forelimb, akin to the human upper limb; in humans, the upper limb is the site of most peripheral nerve lesions.

Original languageEnglish
Article numbere59767
JournalJournal of Visualized Experiments
Issue number158
DOIs
Publication statusPublished - Apr 2020

Keywords

  • Medicine
  • Issue 158
  • median nerve
  • nerve regeneration
  • peripheral nervous system
  • repair
  • rat
  • experimental model
  • surgery
  • functional tests

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