Functional Analysis of PGRP-LA in Drosophila Immunity

M. Gendrin, A. Zaidman-Rémy, N.A. Broderick, J. Paredes, M. Poidevin, A. Roussel, B. Lemaitre

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Abstract

PeptidoGlycan Recognition Proteins (PGRPs) are key regulators of the insect innate antibacterial response. Even if they have been intensively studied, some of them have yet unknown functions. Here, we present a functional analysis of PGRP-LA, an as yet uncharacterized Drosophila PGRP. The PGRP-LA gene is located in cluster with PGRP-LC and PGRP-LF, which encode a receptor and a negative regulator of the Imd pathway, respectively. Structure predictions indicate that PGRP-LA would not bind to peptidoglycan, pointing to a regulatory role of this PGRP. PGRP-LA expression was enriched in barrier epithelia, but low in the fat body. Use of a newly generated PGRP-LA deficient mutant indicates that PGRP-LA is not required for the production of antimicrobial peptides by the fat body in response to a systemic infection. Focusing on the respiratory tract, where PGRP-LA is strongly expressed, we conducted a genome-wide microarray analysis of the tracheal immune response of wild-type, Relish, and PGRP-LA mutant larvae. Comparing our data to previous microarray studies, we report that a majority of genes regulated in the trachea upon infection differ from those induced in the gut or the fat body. Importantly, antimicrobial peptide gene expression was reduced in the tracheae of larvae and in the adult gut of PGRP-LA-deficient Drosophila upon oral bacterial infection. Together, our results suggest that PGRP-LA positively regulates the Imd pathway in barrier epithelia. © 2013 Gendrin et al.
Original languageEnglish
Pages (from-to)Online
Number of pages13
JournalPLoS ONE
Volume8
Issue number7
DOIs
Publication statusPublished - 26 Jul 2013

Keywords

  • peptidoglycan recognition protein
  • article
  • bacterial infection
  • body fat
  • controlled study
  • deletion mutant
  • Drosophila
  • gene
  • gene cluster
  • gene control
  • gene expression
  • gene structure
  • genetic analysis
  • genetic transcription
  • immune response
  • immunity
  • intestine
  • microarray analysis
  • mouth infection
  • nonhuman
  • PGRP LA gene
  • protein function
  • relish gene
  • trachea
  • upregulation
  • Amino Acid Sequence
  • Animals
  • Carrier Proteins
  • Drosophila melanogaster
  • Drosophila Proteins
  • Epithelium
  • Genetic Loci
  • Gram-Negative Bacterial Infections
  • Immunity
  • Larva
  • Molecular Sequence Data
  • Oligonucleotide Array Sequence Analysis
  • Pectobacterium carotovorum
  • Peptidoglycan
  • Protein Binding
  • Protein Isoforms
  • Signal Transduction
  • Trachea
  • Up-Regulation

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  • Cite this

    Gendrin, M., Zaidman-Rémy, A., Broderick, N. A., Paredes, J., Poidevin, M., Roussel, A., & Lemaitre, B. (2013). Functional Analysis of PGRP-LA in Drosophila Immunity. PLoS ONE, 8(7), Online. https://doi.org/10.1371/journal.pone.0069742