TY - JOUR
T1 - FTIR spectroelectrochemical characterization of the Ni-Fe-Se hydrogenase from Desulfovibrio vulgaris Hildenborough
AU - De Lacey, Antonio L.
AU - Gutiérrez-Sánchez, Cristina
AU - Fernández, Víctor M.
AU - Pacheco, Isabel
AU - Pereira, Inês A.C.
PY - 2008/11/1
Y1 - 2008/11/1
N2 - For the first time a complete characterization by infrared spectroscopy of a Ni-Fe-Se hydrogenase in its different redox states is reported. The Ni-Fe-Se hydrogenase was isolated from Desulfovibrio vulgaris Hildenborough. Two different electron paramagnetic resonance silent and air-stable redox states that are not in equilibrium were detected. Upon reduction of these states the catalytically active states Ni-R and Ni-C appear immediately. These states are in redox equilibrium and their formal redox potential has been measured. Putative structural differences between the redox states of the active site of the Ni-Fe-Se hydrogenase are discussed.
AB - For the first time a complete characterization by infrared spectroscopy of a Ni-Fe-Se hydrogenase in its different redox states is reported. The Ni-Fe-Se hydrogenase was isolated from Desulfovibrio vulgaris Hildenborough. Two different electron paramagnetic resonance silent and air-stable redox states that are not in equilibrium were detected. Upon reduction of these states the catalytically active states Ni-R and Ni-C appear immediately. These states are in redox equilibrium and their formal redox potential has been measured. Putative structural differences between the redox states of the active site of the Ni-Fe-Se hydrogenase are discussed.
KW - Hydrogenase
KW - Infrared
KW - Selenocysteine
KW - Spectroelectrochemistry
UR - http://www.scopus.com/inward/record.url?scp=55949104332&partnerID=8YFLogxK
U2 - 10.1007/s00775-008-0412-5
DO - 10.1007/s00775-008-0412-5
M3 - Article
C2 - 18704522
AN - SCOPUS:55949104332
SN - 0949-8257
VL - 13
SP - 1315
EP - 1320
JO - JBIC Journal of Biological Inorganic Chemistry
JF - JBIC Journal of Biological Inorganic Chemistry
IS - 8
ER -