Fractionation of Cynara cardunculus ethanolic extracts using diananofiltration

Teresa Brás, Daniela Rosa, Ana C. Gonçalves, Andreia C. Gomes, Carla Brazinha, Luísa A. Neves, Maria F. Duarte, João G. Crespo

Research output: Contribution to journalArticlepeer-review


Cynara cardunculus (cardoon) leaves are a rich source of bioactive compounds, such as cynaropicrin. In this work, ethanolic pulsed ultrasound assisted extraction (EtPUAE) derived extracts obtained from cardoon leaves were purified by membrane processing and the different fractions obtained were evaluated for their biological potential. A DuraMem® 200 membrane was selected for cynaropicrin recovery and removal of carbohydrates, in a diananofiltration mode. Diananofiltration (after 5 diavolumes) followed by nanofiltration allowed for a removal of 93.0% of glucose and 95.6% of fructose, with a cynaropicrin maximum loss of 13.9%. The fractionated extract revealed a higher biological activity, when tested with a BJ5-ta human normal fibroblast cell line. An integrated process is proposed, comprising an association of EtPUAE with a fractionation by diananofiltration, assuring a pay-back period of 4.58 years, assessed through an economic evaluation.

Original languageEnglish
Article number117856
JournalSeparation and Purification Technology
Publication statusPublished - 1 Feb 2021


  • Biological activity
  • Carbohydrates
  • Cynaropicrin
  • Diananofiltration
  • Economic evaluation
  • Organic solvent nanofiltration


Dive into the research topics of 'Fractionation of Cynara cardunculus ethanolic extracts using diananofiltration'. Together they form a unique fingerprint.

Cite this