Fluorescent reporters for studies of cellular localization of proteins in Staphylococcus aureus

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We have constructed a set of plasmids that allow expression, from their native chromosomal loci, of Staphylococcus aureus proteins fused to one of four different fluorescent proteins (green fluorescent protein [GFP], cyan fluorescent protein [CFP], yellow fluorescent protein [YFP], and mCherry), using two different resistance markers (kanamycin and erythromycin). We have also constructed a plasmid that allows expression of proteins from the ectopic spa locus in the S. aureus chromosome. This toolbox can be used for studies of the localization of proteins in S. aureus, a prominent pathogen in both health care and community settings.
Original languageUnknown
Pages (from-to)4346-4353
JournalApplied and Environmental Microbiology
Volume76
Issue number13
DOIs
Publication statusPublished - 1 Jan 2010

Cite this

@article{f61bfc1aae9c43fd874a521865ad5452,
title = "Fluorescent reporters for studies of cellular localization of proteins in Staphylococcus aureus",
abstract = "We have constructed a set of plasmids that allow expression, from their native chromosomal loci, of Staphylococcus aureus proteins fused to one of four different fluorescent proteins (green fluorescent protein [GFP], cyan fluorescent protein [CFP], yellow fluorescent protein [YFP], and mCherry), using two different resistance markers (kanamycin and erythromycin). We have also constructed a plasmid that allows expression of proteins from the ectopic spa locus in the S. aureus chromosome. This toolbox can be used for studies of the localization of proteins in S. aureus, a prominent pathogen in both health care and community settings.",
keywords = "GRAM-POSITIVE BACTERIA, BACILLUS-SUBTILIS, IDENTIFICATION, FTSZ, EXPRESSION, DNA, SYSTEM, VIRULENCE, DIVISION, RESISTANCE",
author = "Pinho, {Mariana Luisa}",
note = "[Pereira, Pedro M.; Veiga, Helena; Jorge, Ana M. (ITQB)",
year = "2010",
month = "1",
day = "1",
doi = "10.1128/aem.00359-10",
language = "Unknown",
volume = "76",
pages = "4346--4353",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "13",

}

Fluorescent reporters for studies of cellular localization of proteins in Staphylococcus aureus. / Pinho, Mariana Luisa.

In: Applied and Environmental Microbiology, Vol. 76, No. 13, 01.01.2010, p. 4346-4353.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fluorescent reporters for studies of cellular localization of proteins in Staphylococcus aureus

AU - Pinho, Mariana Luisa

N1 - [Pereira, Pedro M.; Veiga, Helena; Jorge, Ana M. (ITQB)

PY - 2010/1/1

Y1 - 2010/1/1

N2 - We have constructed a set of plasmids that allow expression, from their native chromosomal loci, of Staphylococcus aureus proteins fused to one of four different fluorescent proteins (green fluorescent protein [GFP], cyan fluorescent protein [CFP], yellow fluorescent protein [YFP], and mCherry), using two different resistance markers (kanamycin and erythromycin). We have also constructed a plasmid that allows expression of proteins from the ectopic spa locus in the S. aureus chromosome. This toolbox can be used for studies of the localization of proteins in S. aureus, a prominent pathogen in both health care and community settings.

AB - We have constructed a set of plasmids that allow expression, from their native chromosomal loci, of Staphylococcus aureus proteins fused to one of four different fluorescent proteins (green fluorescent protein [GFP], cyan fluorescent protein [CFP], yellow fluorescent protein [YFP], and mCherry), using two different resistance markers (kanamycin and erythromycin). We have also constructed a plasmid that allows expression of proteins from the ectopic spa locus in the S. aureus chromosome. This toolbox can be used for studies of the localization of proteins in S. aureus, a prominent pathogen in both health care and community settings.

KW - GRAM-POSITIVE BACTERIA

KW - BACILLUS-SUBTILIS

KW - IDENTIFICATION

KW - FTSZ

KW - EXPRESSION

KW - DNA

KW - SYSTEM

KW - VIRULENCE

KW - DIVISION

KW - RESISTANCE

U2 - 10.1128/aem.00359-10

DO - 10.1128/aem.00359-10

M3 - Article

VL - 76

SP - 4346

EP - 4353

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 13

ER -