Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina

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Abstract

Two-dimensional (2D) fluorescence spectroscopy was investigated as a monitoring tool for cultivation, harvesting, and effluent treatment of Dunaliella salina with high carotenoid concentration; aiming to improve the production process and minimise costs. Chemometric analysis, namely Principal Component Analysis (PCA) and Projection to Latent Structures (PLS), were used to build models for estimation of cellular concentration, cellular viability, and nitrate concentration in media. The estimations were based on fluorescence excitation-emission matrices (EEMs) acquired directly from algal suspensions. Cell concentration during cultivation and harvesting can be predicted by a single model capturing 92.0% of the variance, and with R2 of 0.92 and 0.97, for training and validation, respectively. Cell viability during harvesting by ultrafiltration was modelled with 79% of variance and R2 of 0.79 for training and 0.73 for validation. Nitrate concentration was successfully predicted during cultivation and permeate treatment using a single model with 81.8% of variance and R2 of 0.82 for training and 0.80 for validation. Therefore, this work demonstrates the strong potential of combining 2D fluorescence and chemometrics for monitoring different processes during microalgae production.

Original languageEnglish
Article number101720
JournalAlgal Research
Volume44
DOIs
Publication statusPublished - 1 Dec 2019

Fingerprint

Dunaliella salina
chemometrics
cell viability
carotenoids
nitrates
fluorescence
monitoring
fluorescence emission spectroscopy
process monitoring
permeates
cells
ultrafiltration
microalgae
effluents
principal component analysis
viability

Keywords

  • Carotenogenesis
  • Dunaliella salina
  • Fluorescence EEMs
  • Membrane harvesting
  • Monitoring

Cite this

@article{67a7c3b9434f41c78264c7d28b71026f,
title = "Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina",
abstract = "Two-dimensional (2D) fluorescence spectroscopy was investigated as a monitoring tool for cultivation, harvesting, and effluent treatment of Dunaliella salina with high carotenoid concentration; aiming to improve the production process and minimise costs. Chemometric analysis, namely Principal Component Analysis (PCA) and Projection to Latent Structures (PLS), were used to build models for estimation of cellular concentration, cellular viability, and nitrate concentration in media. The estimations were based on fluorescence excitation-emission matrices (EEMs) acquired directly from algal suspensions. Cell concentration during cultivation and harvesting can be predicted by a single model capturing 92.0{\%} of the variance, and with R2 of 0.92 and 0.97, for training and validation, respectively. Cell viability during harvesting by ultrafiltration was modelled with 79{\%} of variance and R2 of 0.79 for training and 0.73 for validation. Nitrate concentration was successfully predicted during cultivation and permeate treatment using a single model with 81.8{\%} of variance and R2 of 0.82 for training and 0.80 for validation. Therefore, this work demonstrates the strong potential of combining 2D fluorescence and chemometrics for monitoring different processes during microalgae production.",
keywords = "Carotenogenesis, Dunaliella salina, Fluorescence EEMs, Membrane harvesting, Monitoring",
author = "Marta S{\'a} and Joana Monte and Carla Brazinha and Galinha, {Claudia F.} and Crespo, {Jo{\~a}o G.}",
note = "This work was supported by the Associate Laboratory for Green Chemistry- LAQV which is financed by national funds from FCT / MCTES ( UID/QUI/50006/2019 ), by the European FP7 KBBE project “D-Factory” (contract no. 613870), by KAUST OSR award no. OSR-2016-CPF-2907-05 , and by the follow Fellow grants of FCT / MCTES : SFRH/BPD/95864/2013 , SFRH/BPD/79533/2011 and SFRH/BD/108894/2015 . The authors would like to thank The Marine Biological Association (Devon, UK) and NBT Ltd (Israel).",
year = "2019",
month = "12",
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doi = "10.1016/j.algal.2019.101720",
language = "English",
volume = "44",
journal = "Algal Research",
issn = "2211-9264",
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T1 - Fluorescence coupled with chemometrics for simultaneous monitoring of cell concentration, cell viability and medium nitrate during production of carotenoid-rich Dunaliella salina

AU - Sá, Marta

AU - Monte, Joana

AU - Brazinha, Carla

AU - Galinha, Claudia F.

AU - Crespo, João G.

N1 - This work was supported by the Associate Laboratory for Green Chemistry- LAQV which is financed by national funds from FCT / MCTES ( UID/QUI/50006/2019 ), by the European FP7 KBBE project “D-Factory” (contract no. 613870), by KAUST OSR award no. OSR-2016-CPF-2907-05 , and by the follow Fellow grants of FCT / MCTES : SFRH/BPD/95864/2013 , SFRH/BPD/79533/2011 and SFRH/BD/108894/2015 . The authors would like to thank The Marine Biological Association (Devon, UK) and NBT Ltd (Israel).

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Two-dimensional (2D) fluorescence spectroscopy was investigated as a monitoring tool for cultivation, harvesting, and effluent treatment of Dunaliella salina with high carotenoid concentration; aiming to improve the production process and minimise costs. Chemometric analysis, namely Principal Component Analysis (PCA) and Projection to Latent Structures (PLS), were used to build models for estimation of cellular concentration, cellular viability, and nitrate concentration in media. The estimations were based on fluorescence excitation-emission matrices (EEMs) acquired directly from algal suspensions. Cell concentration during cultivation and harvesting can be predicted by a single model capturing 92.0% of the variance, and with R2 of 0.92 and 0.97, for training and validation, respectively. Cell viability during harvesting by ultrafiltration was modelled with 79% of variance and R2 of 0.79 for training and 0.73 for validation. Nitrate concentration was successfully predicted during cultivation and permeate treatment using a single model with 81.8% of variance and R2 of 0.82 for training and 0.80 for validation. Therefore, this work demonstrates the strong potential of combining 2D fluorescence and chemometrics for monitoring different processes during microalgae production.

AB - Two-dimensional (2D) fluorescence spectroscopy was investigated as a monitoring tool for cultivation, harvesting, and effluent treatment of Dunaliella salina with high carotenoid concentration; aiming to improve the production process and minimise costs. Chemometric analysis, namely Principal Component Analysis (PCA) and Projection to Latent Structures (PLS), were used to build models for estimation of cellular concentration, cellular viability, and nitrate concentration in media. The estimations were based on fluorescence excitation-emission matrices (EEMs) acquired directly from algal suspensions. Cell concentration during cultivation and harvesting can be predicted by a single model capturing 92.0% of the variance, and with R2 of 0.92 and 0.97, for training and validation, respectively. Cell viability during harvesting by ultrafiltration was modelled with 79% of variance and R2 of 0.79 for training and 0.73 for validation. Nitrate concentration was successfully predicted during cultivation and permeate treatment using a single model with 81.8% of variance and R2 of 0.82 for training and 0.80 for validation. Therefore, this work demonstrates the strong potential of combining 2D fluorescence and chemometrics for monitoring different processes during microalgae production.

KW - Carotenogenesis

KW - Dunaliella salina

KW - Fluorescence EEMs

KW - Membrane harvesting

KW - Monitoring

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U2 - 10.1016/j.algal.2019.101720

DO - 10.1016/j.algal.2019.101720

M3 - Article

VL - 44

JO - Algal Research

JF - Algal Research

SN - 2211-9264

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ER -