FISH in Suspension or in Adherent Cells

Francesca Di Pippo, Diogo Queirós, Joana Pereira, Paulo C. Lemos, Luísa S. Serafim, Simona Rossetti

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Fluorescence in situ hybridization (FISH) enables the detection and enumeration of microorganisms in a diversity of samples. Short-length oligonucleotide DNA probes complementary to 16S or 23S rRNA sequences are generally used to target different phylogenetic levels. The protocol for the application of FISH to aggregated or suspended cells in mixed microbial communities is described in this chapter, with a special emphasis on environmental samples.

Original languageEnglish
Title of host publicationFluorescence In-Situ Hybridization (FISH) for Microbial Cells
EditorsN. F. Azevedo, C. Almeida
Place of PublicationNew York, NY
PublisherHumana Press
Number of pages17
ISBN (Electronic)978-1-0716-1115-9
ISBN (Print)978-1-0716-1114-2
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Adherent cells
  • Environmental samples
  • FISH
  • Oligonucleotide probes
  • Suspension cells
  • Unculturable bacteria


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