First report of an Onchocercidae worm infecting Psychodopygus carrerai carrerai sandfly, a putative vector of Leishmania braziliensis in the Amazon

Andreia Fernandes Brilhante, Alessandra Lima de Albuquerque, Abraham Cézar de Brito Rocha, Constância Flávia Junqueira Ayres, Marcelo Henrique Santos Paiva, Márcia Moreira de Ávila, Cristiane de Oliveira Cardoso, Isabel L. Mauricio, Eunice Aparecida Bianchi Galati

Research output: Contribution to journalArticle

Abstract

Sandflies are insects of public health interest due to their role as vectors of parasites of the genus Leishmania, as well as other pathogens. Psychodopygus carrerai carrerai is considered an important sylvatic vector of Leishmania (Viannia) braziliensis in Amazonia. In this study, sandflies were collected in a forested area in the Xapuri municipality, in the State of Acre (Northern Brazil). Two Ps. carrerai carrerai females were found parasitized with a larval form of a filarial worm, one in the labium of the proboscis, the other after the head was squashed, suggesting they were infective larvae. Sandflies were identified through morphological characters as well as amplification and sequencing of the cytochrome oxidase gene (COI). This was the first sequence obtained for Ps. carrerai carrerai for this marker. The obtained nematodes were also characterized through direct sequencing of a fragment of COI and 12S genes, both mitochondrial, and ITS1, a nuclear marker. Phylogenetic analyses revealed that the filarial nematodes belong to a species without sequences for these markers in the database, part of family Onchocercidade and closely related to genus Onchocerca (12S tree). Although sandfly infection with nematodes including members of the Onchocercidae has been reported in the Old World, this is the first report of sandfly infection by a member of the Onchocercidae family in the New World, to the best of our knowledge. Considering that the phylogenetic relationships and location in the insect, it can be expected that this is a parasite of mammals and the transmission cycle should be clarified.

Original languageEnglish
Article number15246
Pages (from-to)15246-15255
Number of pages9
JournalScientific Reports
VolumeVol. 10
Issue numbern.º 1
DOIs
Publication statusPublished - 17 Sep 2020

Keywords

  • Entomology
  • Parasitic infection

UN Sustainable Development Goals (SDGs)

  • SDG 3 - Good Health and Well-Being
  • SDG 15 - Life on Land

Fingerprint Dive into the research topics of 'First report of an Onchocercidae worm infecting Psychodopygus carrerai carrerai sandfly, a putative vector of Leishmania braziliensis in the Amazon'. Together they form a unique fingerprint.

Cite this