First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested-PCR

Ana S. Faria; Maria das Neves Paiva-Cardoso; Mónica Nunes; Teresa Carreira; Hélia M. Vale-Gonçalves; Octávia Veloso; Catarina Coelho; João A. Cabral; Madalena Vieira-Pinto; Maria L. Vieira

doi:10.1007/s10393-014-0973-4

First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested-PCR

Ana S. Faria, Maria das Neves Paiva-Cardoso, Mónica Nunes, Teresa Carreira, Hélia M. Vale-Gonçalves, Octávia Veloso, Catarina Coelho, João A. Cabral, Madalena Vieira-Pinto, Maria L. Vieira

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Lyme borreliosis is the most common tick-borne zoonosis in the northern hemisphere. Several vertebrates are crucial in the epidemiological cycle of Borrelia burgdorferi sensu lato, but the role of wild boar as a reservoir is still unknown. Sera were collected from 90 wild boars shot in the Trás-os-Montes region, Northern Portugal (hunting season 2011/2012). In this study, Borrelia DNA was detected for the first time by nested-PCR in three different sera, suggesting that the wild boar may be a potential reservoir for this spirochete. Sequencing results show 100% similarity with Borrelia afzelii. Further studies are needed to evaluate the public health risks associated with boar hunting.

Original language	English
Pages (from-to)	183-187
Number of pages	5
Journal	EcoHealth
Volume	12
Issue number	1
DOIs	https://doi.org/10.1007/s10393-014-0973-4
Publication status	Published - 1 Mar 2015

Keywords

Borrelia burgdorferi sensu lato
fla gene
Lyme borreliosis
Northern Portugal
One Health
PCR
wild boar
zoonosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10393-014-0973-4

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title = "First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested-PCR",

abstract = "Lyme borreliosis is the most common tick-borne zoonosis in the northern hemisphere. Several vertebrates are crucial in the epidemiological cycle of Borrelia burgdorferi sensu lato, but the role of wild boar as a reservoir is still unknown. Sera were collected from 90 wild boars shot in the Tr{\'a}s-os-Montes region, Northern Portugal (hunting season 2011/2012). In this study, Borrelia DNA was detected for the first time by nested-PCR in three different sera, suggesting that the wild boar may be a potential reservoir for this spirochete. Sequencing results show 100% similarity with Borrelia afzelii. Further studies are needed to evaluate the public health risks associated with boar hunting.",

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AU - Faria, Ana S.

AU - Paiva-Cardoso, Maria das Neves

AU - Nunes, Mónica

AU - Carreira, Teresa

AU - Vale-Gonçalves, Hélia M.

AU - Veloso, Octávia

AU - Coelho, Catarina

AU - Cabral, João A.

AU - Vieira-Pinto, Madalena

AU - Vieira, Maria L.

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N2 - Lyme borreliosis is the most common tick-borne zoonosis in the northern hemisphere. Several vertebrates are crucial in the epidemiological cycle of Borrelia burgdorferi sensu lato, but the role of wild boar as a reservoir is still unknown. Sera were collected from 90 wild boars shot in the Trás-os-Montes region, Northern Portugal (hunting season 2011/2012). In this study, Borrelia DNA was detected for the first time by nested-PCR in three different sera, suggesting that the wild boar may be a potential reservoir for this spirochete. Sequencing results show 100% similarity with Borrelia afzelii. Further studies are needed to evaluate the public health risks associated with boar hunting.

AB - Lyme borreliosis is the most common tick-borne zoonosis in the northern hemisphere. Several vertebrates are crucial in the epidemiological cycle of Borrelia burgdorferi sensu lato, but the role of wild boar as a reservoir is still unknown. Sera were collected from 90 wild boars shot in the Trás-os-Montes region, Northern Portugal (hunting season 2011/2012). In this study, Borrelia DNA was detected for the first time by nested-PCR in three different sera, suggesting that the wild boar may be a potential reservoir for this spirochete. Sequencing results show 100% similarity with Borrelia afzelii. Further studies are needed to evaluate the public health risks associated with boar hunting.

KW - Borrelia burgdorferi sensu lato

KW - fla gene

KW - Lyme borreliosis

KW - Northern Portugal

KW - One Health

KW - PCR

KW - wild boar

KW - zoonosis

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First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested-PCR

Abstract

Keywords

UN SDGs

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