Fifty years of sperm cell isolations: from structural to omic studies

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Abstract

The fusion of male and female gametes is a fundamental process in the perpetuation and diversification of species. During the last 50 years, significant efforts have been made to isolate and characterize sperm cells from flowering plants, and to identify how these cells interact with female gametes to achieve double fertilization. The first techniques and analytical approaches not only provided structural and biochemical characterizations of plant sperm cells but also paved the way for in vitro fertilization studies. Further technological advances then led to unique insights into sperm biology at the transcriptomic, proteomic, and epigenetic level. Starting with a historical overview of sperm cell isolation techniques, we provide examples of how these contributed to create our current knowledge of sperm cell biology, and point out remaining challenges.

Original languageEnglish
Pages (from-to)3449-3461
Number of pages13
JournalJournal of Experimental Botany
Volume74
Issue number12
DOIs
Publication statusPublished - 27 Jun 2023

Keywords

  • Epigenomics
  • in vitro fertilization
  • isolation techniques
  • pollen
  • proteomics
  • scRNA-seq
  • sperm cells
  • transcriptomics

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