TY - JOUR
T1 - Feeding strategies to optimize vanillin production by Amycolatopsis sp. ATCC 39116
AU - Valério, Rita
AU - Bernardino, Ana R. S.
AU - Torres, Criatiana A. V.
AU - Brazinha, Carla
AU - Tavares, Leonor
AU - Crespo, João G.
AU - Reis, Maria A. M.
N1 - info:eu-repo/grantAgreement/FCT/5876/147218/PT#
FCT/MEC, Portugal, is acknowledged for the PhD Fellows grants SFRH/BD/138011/2018 and PD/BDE/113543/2015.
Applied Molecular Biosciences Unit- UCIBIO which is financed by national funds from FCT/MCTES (UID/Multi/04378/2019);
co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDE R - 007265).
PY - 2021/4
Y1 - 2021/4
N2 - The growing consumer demand for natural products led to an increasing interest in vanillin production by biotechnological routes. In this work, the biotechnological vanillin production by Amycolatopsis sp. ATCC 39116 is studied using ferulic acid as precursor, aiming to achieve maximized vanillin productivities. During biotech-vanillin production, the effects of glucose, vanillin and ferulic acid concentrations in the broth proved to be relevant for vanillin productivity. Concerning glucose, its presence in the broth during the production phase avoids vanillin conversion to vanillic acid and, consequently, increases vanillin production. To avoid the accumulation of vanillin up to a toxic concentration level, a multiple-pulse-feeding strategy is implemented, with intercalated vanillin removal from the broth and biomass recovery. This strategy turned out fruitful, leading to 0.46 g L−1 h−1 volumetric productivity of vanillin of and a production yield of 0.69 gvanillin gferulicacid−1, which are among the highest values reported in the literature for non-modified bacteria.
AB - The growing consumer demand for natural products led to an increasing interest in vanillin production by biotechnological routes. In this work, the biotechnological vanillin production by Amycolatopsis sp. ATCC 39116 is studied using ferulic acid as precursor, aiming to achieve maximized vanillin productivities. During biotech-vanillin production, the effects of glucose, vanillin and ferulic acid concentrations in the broth proved to be relevant for vanillin productivity. Concerning glucose, its presence in the broth during the production phase avoids vanillin conversion to vanillic acid and, consequently, increases vanillin production. To avoid the accumulation of vanillin up to a toxic concentration level, a multiple-pulse-feeding strategy is implemented, with intercalated vanillin removal from the broth and biomass recovery. This strategy turned out fruitful, leading to 0.46 g L−1 h−1 volumetric productivity of vanillin of and a production yield of 0.69 gvanillin gferulicacid−1, which are among the highest values reported in the literature for non-modified bacteria.
KW - Biotech vanillin
KW - Ferulic acid
KW - Pulse feeding strategy
UR - http://www.scopus.com/inward/record.url?scp=85098708900&partnerID=8YFLogxK
U2 - 10.1007/s00449-020-02482-7
DO - 10.1007/s00449-020-02482-7
M3 - Article
C2 - 33389106
AN - SCOPUS:85098708900
SN - 1615-7591
VL - 44
SP - 737
EP - 747
JO - Bioprocess and Biosystems Engineering
JF - Bioprocess and Biosystems Engineering
IS - 4
ER -