Fast nucleotide identification through fingerprinting using gold nanoparticle-based surface-assisted laser desorption/ionisation

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We report a method centred on gold nanoparticle-based surface-assisted laser desorption/ionisation for analysis of deoxynucleotides and alkylated nucleobases. Gold nanoparticles allow for enhanced analysis capability by eliminating undesired signature peaks; thus more elegant mass spectra can be attained that allow identification by nucleotide mass fingerprint. The resulting fingerprinting patterns on the spectra are compared and associated with the presence of different nucleotides in the sample. This method can be easily extended to modified nucleotides implicated in genome lesions due to exposure to environment chemicals, such as DNA adducts (e.g. guanine adducts). The use of gold nanoparticles for surface-assisted laser desorption/ionisation can be an useful tool to resolve common issues of background noise when analysing nucleic acids samples.
Original languageUnknown
Pages (from-to)417-21
JournalTalanta
Volume105
Issue numberNA
DOIs
Publication statusPublished - 1 Jan 2013

Keywords

    Cite this

    @article{b469a18b08ac49fb83d4fb1bb991e382,
    title = "Fast nucleotide identification through fingerprinting using gold nanoparticle-based surface-assisted laser desorption/ionisation",
    abstract = "We report a method centred on gold nanoparticle-based surface-assisted laser desorption/ionisation for analysis of deoxynucleotides and alkylated nucleobases. Gold nanoparticles allow for enhanced analysis capability by eliminating undesired signature peaks; thus more elegant mass spectra can be attained that allow identification by nucleotide mass fingerprint. The resulting fingerprinting patterns on the spectra are compared and associated with the presence of different nucleotides in the sample. This method can be easily extended to modified nucleotides implicated in genome lesions due to exposure to environment chemicals, such as DNA adducts (e.g. guanine adducts). The use of gold nanoparticles for surface-assisted laser desorption/ionisation can be an useful tool to resolve common issues of background noise when analysing nucleic acids samples.",
    keywords = "Glycidamide, Gold nanoparticles, DNA adducts, SALDI-TOF, Nucleotides",
    author = "Capelo, {Jos{\'e} Luis} and Baptista, {Pedro Miguel Ribeiro Viana}",
    note = "Sem PDF",
    year = "2013",
    month = "1",
    day = "1",
    doi = "10.1016/j.talanta.2012.10.072",
    language = "Unknown",
    volume = "105",
    pages = "417--21",
    journal = "Talanta",
    issn = "0039-9140",
    publisher = "Elsevier",
    number = "NA",

    }

    TY - JOUR

    T1 - Fast nucleotide identification through fingerprinting using gold nanoparticle-based surface-assisted laser desorption/ionisation

    AU - Capelo, José Luis

    AU - Baptista, Pedro Miguel Ribeiro Viana

    N1 - Sem PDF

    PY - 2013/1/1

    Y1 - 2013/1/1

    N2 - We report a method centred on gold nanoparticle-based surface-assisted laser desorption/ionisation for analysis of deoxynucleotides and alkylated nucleobases. Gold nanoparticles allow for enhanced analysis capability by eliminating undesired signature peaks; thus more elegant mass spectra can be attained that allow identification by nucleotide mass fingerprint. The resulting fingerprinting patterns on the spectra are compared and associated with the presence of different nucleotides in the sample. This method can be easily extended to modified nucleotides implicated in genome lesions due to exposure to environment chemicals, such as DNA adducts (e.g. guanine adducts). The use of gold nanoparticles for surface-assisted laser desorption/ionisation can be an useful tool to resolve common issues of background noise when analysing nucleic acids samples.

    AB - We report a method centred on gold nanoparticle-based surface-assisted laser desorption/ionisation for analysis of deoxynucleotides and alkylated nucleobases. Gold nanoparticles allow for enhanced analysis capability by eliminating undesired signature peaks; thus more elegant mass spectra can be attained that allow identification by nucleotide mass fingerprint. The resulting fingerprinting patterns on the spectra are compared and associated with the presence of different nucleotides in the sample. This method can be easily extended to modified nucleotides implicated in genome lesions due to exposure to environment chemicals, such as DNA adducts (e.g. guanine adducts). The use of gold nanoparticles for surface-assisted laser desorption/ionisation can be an useful tool to resolve common issues of background noise when analysing nucleic acids samples.

    KW - Glycidamide

    KW - Gold nanoparticles

    KW - DNA adducts

    KW - SALDI-TOF

    KW - Nucleotides

    U2 - 10.1016/j.talanta.2012.10.072

    DO - 10.1016/j.talanta.2012.10.072

    M3 - Article

    VL - 105

    SP - 417

    EP - 421

    JO - Talanta

    JF - Talanta

    SN - 0039-9140

    IS - NA

    ER -