Fast nucleotide identification through fingerprinting using gold nanoparticle-based surface-assisted laser desorption/ionisation

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Abstract

We report a method centred on gold nanoparticle-based surface-assisted laser desorption/ionisation for analysis of deoxynucleotides and alkylated nucleobases. Gold nanoparticles allow for enhanced analysis capability by eliminating undesired signature peaks; thus more elegant mass spectra can be attained that allow identification by nucleotide mass fingerprint. The resulting fingerprinting patterns on the spectra are compared and associated with the presence of different nucleotides in the sample. This method can be easily extended to modified nucleotides implicated in genome lesions due to exposure to environment chemicals, such as DNA adducts (e.g. guanine adducts). The use of gold nanoparticles for surface-assisted laser desorption/ionisation can be an useful tool to resolve common issues of background noise when analysing nucleic acids samples.
Original languageUnknown
Pages (from-to)417-21
JournalTalanta
Volume105
Issue numberNA
DOIs
Publication statusPublished - 1 Jan 2013

Cite this

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title = "Fast nucleotide identification through fingerprinting using gold nanoparticle-based surface-assisted laser desorption/ionisation",
abstract = "We report a method centred on gold nanoparticle-based surface-assisted laser desorption/ionisation for analysis of deoxynucleotides and alkylated nucleobases. Gold nanoparticles allow for enhanced analysis capability by eliminating undesired signature peaks; thus more elegant mass spectra can be attained that allow identification by nucleotide mass fingerprint. The resulting fingerprinting patterns on the spectra are compared and associated with the presence of different nucleotides in the sample. This method can be easily extended to modified nucleotides implicated in genome lesions due to exposure to environment chemicals, such as DNA adducts (e.g. guanine adducts). The use of gold nanoparticles for surface-assisted laser desorption/ionisation can be an useful tool to resolve common issues of background noise when analysing nucleic acids samples.",
keywords = "Glycidamide, Gold nanoparticles, DNA adducts, SALDI-TOF, Nucleotides",
author = "Capelo, {Jos{\'e} Luis} and Baptista, {Pedro Miguel Ribeiro Viana}",
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T1 - Fast nucleotide identification through fingerprinting using gold nanoparticle-based surface-assisted laser desorption/ionisation

AU - Capelo, José Luis

AU - Baptista, Pedro Miguel Ribeiro Viana

N1 - Sem PDF

PY - 2013/1/1

Y1 - 2013/1/1

N2 - We report a method centred on gold nanoparticle-based surface-assisted laser desorption/ionisation for analysis of deoxynucleotides and alkylated nucleobases. Gold nanoparticles allow for enhanced analysis capability by eliminating undesired signature peaks; thus more elegant mass spectra can be attained that allow identification by nucleotide mass fingerprint. The resulting fingerprinting patterns on the spectra are compared and associated with the presence of different nucleotides in the sample. This method can be easily extended to modified nucleotides implicated in genome lesions due to exposure to environment chemicals, such as DNA adducts (e.g. guanine adducts). The use of gold nanoparticles for surface-assisted laser desorption/ionisation can be an useful tool to resolve common issues of background noise when analysing nucleic acids samples.

AB - We report a method centred on gold nanoparticle-based surface-assisted laser desorption/ionisation for analysis of deoxynucleotides and alkylated nucleobases. Gold nanoparticles allow for enhanced analysis capability by eliminating undesired signature peaks; thus more elegant mass spectra can be attained that allow identification by nucleotide mass fingerprint. The resulting fingerprinting patterns on the spectra are compared and associated with the presence of different nucleotides in the sample. This method can be easily extended to modified nucleotides implicated in genome lesions due to exposure to environment chemicals, such as DNA adducts (e.g. guanine adducts). The use of gold nanoparticles for surface-assisted laser desorption/ionisation can be an useful tool to resolve common issues of background noise when analysing nucleic acids samples.

KW - Glycidamide

KW - Gold nanoparticles

KW - DNA adducts

KW - SALDI-TOF

KW - Nucleotides

U2 - 10.1016/j.talanta.2012.10.072

DO - 10.1016/j.talanta.2012.10.072

M3 - Article

VL - 105

SP - 417

EP - 421

JO - Talanta

JF - Talanta

SN - 0039-9140

IS - NA

ER -