Fast human serum profiling through chemical depletion coupled to gold-nanoparticle-assisted protein separation

Rubén López-Cortés, Elisabete Oliveira, Cristina Núñez, Carlos Lodeiro, María Páez De La Cadena, Florentino Fdez-Riverola, H. López-Fernández, Miguel Reboiro-Jato, Daniel Glez-Peña, José Luis Capelo, Hugo M. Santos

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The use of chemical protein depletion in conjunction with gold-based nanoparticles for fast matrix assisted laser desoption ionization time of flight mass spectrometry-based human serum profiling was assessed. The following variables influencing the process were optimized: (i) amount of nanoparticles, (ii) sample pH, (iii) amount of protein and (iv) incubation time. pH was found the most important factor to be controlled, with an optimum range comprised between 5.8 and 6.4. The minimum incubation time to obtain an adequate profiling was 30 min. Using this approach, serum from five patients with lymphoma, five patients with myeloma and from two healthy volunteers were correctly classified using Principal component analysis.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalTalanta
Volume100
DOIs
Publication statusPublished - 15 Oct 2012

Keywords

  • Chemical depletion
  • MALDI
  • Medical diagnostics
  • Nanomedicine
  • Nanoparticles
  • Serum profile

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