@article{a4a725bcf7b64d0b89f101618cecda7c,
title = "Fast human serum profiling through chemical depletion coupled to gold-nanoparticle-assisted protein separation",
abstract = "The use of chemical protein depletion in conjunction with gold-based nanoparticles for fast matrix assisted laser desoption ionization time of flight mass spectrometry-based human serum profiling was assessed. The following variables influencing the process were optimized: (i) amount of nanoparticles, (ii) sample pH, (iii) amount of protein and (iv) incubation time. pH was found the most important factor to be controlled, with an optimum range comprised between 5.8 and 6.4. The minimum incubation time to obtain an adequate profiling was 30 min. Using this approach, serum from five patients with lymphoma, five patients with myeloma and from two healthy volunteers were correctly classified using Principal component analysis.",
keywords = "Chemical depletion, MALDI, Medical diagnostics, Nanomedicine, Nanoparticles, Serum profile",
author = "Rub{\'e}n L{\'o}pez-Cort{\'e}s and Elisabete Oliveira and Cristina N{\'u}{\~n}ez and Carlos Lodeiro and {De La Cadena}, {Mar{\'i}a P{\'a}ez} and Florentino Fdez-Riverola and H. L{\'o}pez-Fern{\'a}ndez and Miguel Reboiro-Jato and Daniel Glez-Pe{\~n}a and {Luis Capelo}, Jos{\'e} and Santos, {Hugo M.}",
note = "H.M. Santos, E. Oliveira and Cristina Nunez acknowledge the post-doctoral grant SRFH/BPD/73997/2010, SFRH/BPD/72557/2010 and SFRH/BPD/65367/2009, respectively, provided by Fundacao para a Ciencia e a Tecnologia-Ministerio da Educacao e Ciencia, FCT-MEC, Portugal. J.L Capelo and C. Lodeiro are grateful to the Xunta de Galicia (Spain) for the program Isidro Parga Pondal and for financial support provided under projects 09CSA043383PR and 10CSA383009PR-2010.",
year = "2012",
month = oct,
day = "15",
doi = "10.1016/j.talanta.2012.08.020",
language = "English",
volume = "100",
pages = "239--245",
journal = "Talanta",
issn = "0039-9140",
publisher = "Elsevier",
}
