Expression profiling of castanea genes during resistant and susceptible interactions with the oomycete pathogen phytophthora cinnamomi reveal possible mechanisms of immunity

Carmen Santos, Sofia Duarte, Sara Tedesco, Pedro Fevereiro, Rita L. Costa

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The most dangerous pathogen affecting the production of chestnuts is Phytophthora cinnamomi a hemibiotrophic that causes root rot, also known as ink disease. Little information has been acquired in chestnut on the molecular defense strategies against this pathogen. The expression of eight candidate genes potentially involved in the defense to P. Cinnamomi was quantified by digital PCR in Castanea genotypes showing different susceptibility to the pathogen. Seven of the eight candidate genes displayed differentially expressed levels depending on genotype and time-point after inoculation. Cast_Gnk2-like revealed to be the most expressed gene across all experiments and the one that best discriminates between susceptible and resistant genotypes. Our data suggest that the pre-formed defenses are crucial for the resistance of C. crenata to P. Cinnamomi. A lower and delayed expression of the eight studied genes was found in the susceptible Castanea sativa, which may be related with the establishment and spread of the disease in this species. A working model integrating the obtained results is presented.

Original languageEnglish
Article number515
JournalFrontiers in Plant Science
Volume8
DOIs
Publication statusPublished - 11 Apr 2017

Keywords

  • Castanea
  • Digital PCR
  • Ink disease
  • Phytophthora cinnamomi
  • Plant biotic interactions

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