TY - JOUR
T1 - Expression of Extracellular Vesicle PIWI-Interacting RNAs Throughout hiPSC-Cardiomyocyte Differentiation
AU - Louro, Ana F.
AU - Virgolini, Nikolaus
AU - Paiva, Marta A.
AU - Isidro, Inês A.
AU - Alves, Paula M.
AU - Gomes-Alves, Patrícia
AU - Serra, Margarida
N1 - Funding Information:
This work was supported by European Union’s funded project BRAV3 (H2020, ID:874827); EU Interreg Sudoe ‐ funded project CardioPatch (SOE4/P1/E1063); Fundação para a Ciência e Tecnologia (FCT)-funded projects NETDIAMOND (SAICTPAC/0047/2015) and MetaCardio (PTDC/BTMSAL/32566/2017). This work was funded by Fundação para a Ciência e Tecnologia/Ministêrio da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020) and the Associate Laboratory LS4FUTURE (LA/P/0087/2020). AL was funded by FCT under grant PD/BD/139078/2018. NV has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No. 813453.
Publisher Copyright:
Copyright © 2022 Louro, Virgolini, Paiva, Isidro, Alves, Gomes-Alves and Serra.
PY - 2022/6/16
Y1 - 2022/6/16
N2 - Extracellular Vesicles (EV) play a critical role in the regulation of regenerative processes in wounded tissues by mediating cell-to-cell communication. Multiple RNA species have been identified in EV, although their function still lacks understanding. We previously characterized the miRNA content of EV secreted over hiPSC-cardiomyocyte differentiation and found a distinct miRNA expression in hiPSC-EV driving its in vitro bioactivity. In this work, we investigated the piRNA profiles of EV derived from key stages of the hiPSC-CM differentiation and maturation, i.e., from hiPSC (hiPSC-EV), cardiac progenitors (CPC-EV), immature (CMi-EV), and mature (CMm-EV) cardiomyocytes, demonstrating that EV-piRNA expression differs greatly from the miRNA profiles we previously identified. Only four piRNA were significantly deregulated in EV, one in hiPSC-EV, and three in CPC-EV, as determined by differential expression analysis on small RNA-seq data. Our results provide a valuable source of information for further studies aiming at defining the role of piRNA in the bioactivity and therapeutic potential of EV.
AB - Extracellular Vesicles (EV) play a critical role in the regulation of regenerative processes in wounded tissues by mediating cell-to-cell communication. Multiple RNA species have been identified in EV, although their function still lacks understanding. We previously characterized the miRNA content of EV secreted over hiPSC-cardiomyocyte differentiation and found a distinct miRNA expression in hiPSC-EV driving its in vitro bioactivity. In this work, we investigated the piRNA profiles of EV derived from key stages of the hiPSC-CM differentiation and maturation, i.e., from hiPSC (hiPSC-EV), cardiac progenitors (CPC-EV), immature (CMi-EV), and mature (CMm-EV) cardiomyocytes, demonstrating that EV-piRNA expression differs greatly from the miRNA profiles we previously identified. Only four piRNA were significantly deregulated in EV, one in hiPSC-EV, and three in CPC-EV, as determined by differential expression analysis on small RNA-seq data. Our results provide a valuable source of information for further studies aiming at defining the role of piRNA in the bioactivity and therapeutic potential of EV.
KW - Akt
KW - extracellular vesicles
KW - hiPSC-cardiomyocyte
KW - piRNA
KW - small RNA-seq
UR - http://www.scopus.com/inward/record.url?scp=85133506579&partnerID=8YFLogxK
U2 - 10.3389/fphys.2022.926528
DO - 10.3389/fphys.2022.926528
M3 - Article
AN - SCOPUS:85133506579
SN - 1664-042X
VL - 13
JO - Frontiers in Physiology
JF - Frontiers in Physiology
M1 - 926528
ER -