Expression of Extracellular Vesicle PIWI-Interacting RNAs Throughout hiPSC-Cardiomyocyte Differentiation

Ana F. Louro, Nikolaus Virgolini, Marta A. Paiva, Inês A. Isidro, Paula M. Alves, Patrícia Gomes-Alves, Margarida Serra

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Extracellular Vesicles (EV) play a critical role in the regulation of regenerative processes in wounded tissues by mediating cell-to-cell communication. Multiple RNA species have been identified in EV, although their function still lacks understanding. We previously characterized the miRNA content of EV secreted over hiPSC-cardiomyocyte differentiation and found a distinct miRNA expression in hiPSC-EV driving its in vitro bioactivity. In this work, we investigated the piRNA profiles of EV derived from key stages of the hiPSC-CM differentiation and maturation, i.e., from hiPSC (hiPSC-EV), cardiac progenitors (CPC-EV), immature (CMi-EV), and mature (CMm-EV) cardiomyocytes, demonstrating that EV-piRNA expression differs greatly from the miRNA profiles we previously identified. Only four piRNA were significantly deregulated in EV, one in hiPSC-EV, and three in CPC-EV, as determined by differential expression analysis on small RNA-seq data. Our results provide a valuable source of information for further studies aiming at defining the role of piRNA in the bioactivity and therapeutic potential of EV.

Original languageEnglish
Article number926528
JournalFrontiers in Physiology
Volume13
DOIs
Publication statusPublished - 16 Jun 2022

Keywords

  • Akt
  • extracellular vesicles
  • hiPSC-cardiomyocyte
  • piRNA
  • small RNA-seq

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