TY - JOUR
T1 - Exploring the gas access routes in a [NiFeSe] hydrogenase using crystals pressurized with krypton and oxygen
AU - Zacarias, Sónia
AU - Temporão, Adriana
AU - Carpentier, Philippe
AU - van der Linden, Peter
AU - Pereira, Inês A.C.
AU - Matias, Pedro M.
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Hydrogenases are metalloenzymes that catalyse both H2 evolution and uptake. They are gas-processing enzymes with deeply buried active sites, so the gases diffuse through channels that connect the active site to the protein surface. The [NiFeSe] hydrogenases are a special class of hydrogenases containing a selenocysteine as a nickel ligand; they are more catalytically active and less O2-sensitive than standard [NiFe] hydrogenases. Characterisation of the channel system of hydrogenases is important to understand how the inhibitor oxygen reaches the active site to cause oxidative damage. To this end, crystals of Desulfovibrio vulgaris Hildenborough [NiFeSe] hydrogenase were pressurized with krypton and oxygen, and a method for tracking labile O2 molecules was developed, for mapping a hydrophobic channel system similar to that of the [NiFe] enzymes as the major route for gas diffusion.
AB - Hydrogenases are metalloenzymes that catalyse both H2 evolution and uptake. They are gas-processing enzymes with deeply buried active sites, so the gases diffuse through channels that connect the active site to the protein surface. The [NiFeSe] hydrogenases are a special class of hydrogenases containing a selenocysteine as a nickel ligand; they are more catalytically active and less O2-sensitive than standard [NiFe] hydrogenases. Characterisation of the channel system of hydrogenases is important to understand how the inhibitor oxygen reaches the active site to cause oxidative damage. To this end, crystals of Desulfovibrio vulgaris Hildenborough [NiFeSe] hydrogenase were pressurized with krypton and oxygen, and a method for tracking labile O2 molecules was developed, for mapping a hydrophobic channel system similar to that of the [NiFe] enzymes as the major route for gas diffusion.
KW - Gas channels
KW - High-pressure derivatization
KW - Hydrogenase
KW - Selenium
UR - http://www.scopus.com/inward/record.url?scp=85089970458&partnerID=8YFLogxK
U2 - 10.1007/s00775-020-01814-y
DO - 10.1007/s00775-020-01814-y
M3 - Article
AN - SCOPUS:85089970458
SN - 0949-8257
VL - 25
SP - 863
EP - 874
JO - JBIC Journal of Biological Inorganic Chemistry
JF - JBIC Journal of Biological Inorganic Chemistry
IS - 6
ER -