TY - JOUR
T1 - Exploring the biological properties and bioaccessibility of orange peel extracts using deep eutectic systems
AU - Viñas-Ospino, Adriana
AU - Sá-Nogueira, Isabel
AU - Duarte, Ana Rita
AU - López-Malo, Daniel
AU - Esteve, María José
AU - Frígola, Ana
AU - Blesa, Jesús
AU - Paiva, Alexandre
N1 - Funding Information:
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT#
info:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0140%2F2020/PT#
This work was financially supported by the Ministry of Science and Innovation (Spain) -State Research Agency (PID- 2019-111331RB-I00/AEI/10.13039/501100011033) and by “Generación Bicentenario” scholarship from the Ministry of Education of the Republic of Peru (PRONABEC). Agricultural Cooperative Sant Bernat from Carlet, Spain, donated the raw materials. COST Action: “Greenering-CA18224”, Short Term Scientific Mission.
© 2024 The Authors. Published by Elsevier Ltd.
PY - 2024/10
Y1 - 2024/10
N2 - The ability to recover bio-compounds from by-products offers a significant chance for their use as bio-additives in the food industry. Carotenoids are known for their properties as antioxidants, antimicrobials, and food colorants. In the present study, the potential of hydrophobic natural deep eutectic systems (HDES) for the revalorization of orange peels was explored. Extracts were obtained, and their biological properties were evaluated in vitro, such as cytotoxicity, antiproliferative capacity, antioxidant capacity, and antimicrobial potential against Staphylococcus aureus and Escherichia coli. To support the potential application of orange peel – HDES extract as a functional ingredient or food additive, INFOGEST in vitro static digestion was performed. The results showed that Octanoic acid: Proline (C8:Pro) extract and Menthol: Eucalypthol (Me:Eu) had higher selective index meaning that these HDES have a higher selectivity towards human colorectal adenocarcinoma cell lines. Also, C8:Pro showed a higher carotenoid extraction yield extracting 168 μg/100 g of lutein with ORAC value of 34.8 mM TE. In general, just C8:Pro extract showed inhibition against both bacteria. Bioaccessibility of β-cryptoxanthin was enhanced in C8:Pro extract in 10.1%, indicating high stability during gastrointestinal digestion. These results reveal that orange peel – HDES are promising to be of use in industrial food applications.
AB - The ability to recover bio-compounds from by-products offers a significant chance for their use as bio-additives in the food industry. Carotenoids are known for their properties as antioxidants, antimicrobials, and food colorants. In the present study, the potential of hydrophobic natural deep eutectic systems (HDES) for the revalorization of orange peels was explored. Extracts were obtained, and their biological properties were evaluated in vitro, such as cytotoxicity, antiproliferative capacity, antioxidant capacity, and antimicrobial potential against Staphylococcus aureus and Escherichia coli. To support the potential application of orange peel – HDES extract as a functional ingredient or food additive, INFOGEST in vitro static digestion was performed. The results showed that Octanoic acid: Proline (C8:Pro) extract and Menthol: Eucalypthol (Me:Eu) had higher selective index meaning that these HDES have a higher selectivity towards human colorectal adenocarcinoma cell lines. Also, C8:Pro showed a higher carotenoid extraction yield extracting 168 μg/100 g of lutein with ORAC value of 34.8 mM TE. In general, just C8:Pro extract showed inhibition against both bacteria. Bioaccessibility of β-cryptoxanthin was enhanced in C8:Pro extract in 10.1%, indicating high stability during gastrointestinal digestion. These results reveal that orange peel – HDES are promising to be of use in industrial food applications.
KW - Bioaccessibility
KW - Bioactivity
KW - Carotenoids
KW - Deep eutectic solvents
KW - Green extraction
KW - Orange peels
UR - http://www.scopus.com/inward/record.url?scp=85197582173&partnerID=8YFLogxK
U2 - 10.1016/j.fbio.2024.104684
DO - 10.1016/j.fbio.2024.104684
M3 - Article
AN - SCOPUS:85197582173
SN - 2212-4292
VL - 61
JO - Food Bioscience
JF - Food Bioscience
M1 - 104684
ER -