Exploring analytical proteomics platforms toward the definition of human cardiac stem cells receptome

Patrícia Gomes-Alves, Margarida Serra, Ana Catarina Montes, Luis R.-Borlado, Juan A. López, Jesús Vázquez, Manuel J T Carrondo, António Bernad, Paula M. Alves

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9 Citations (Scopus)

Abstract

Human cardiac stem cells (hCSC) express a portfolio of plasma membrane receptors that are involved in the regulatory auto/paracrine feedback loop mechanism of activation of these cells, and consequently contribute to myocardial regeneration. In order to attain a comprehensive description of hCSC receptome and overcoming the inability demonstrated by other technologies applied in receptor identification, mainly due to the transmembrane nature, high hydrophobic character and relative low concentration of these proteins, we have exploited and improved a proteomics workflow. This approach was based on the enrichment of hCSC plasma membrane fraction and addition of prefractionation steps prior to MS analysis. More than 100 plasma membrane receptors were identified. The data reported herein constitute a valuable source of information to further understand cardiac stem cells activation mechanisms and the subsequent cardiac repair process. All MS data have been deposited in the ProteomeXchange with identifier PXD001117 (http://proteomecentral.proteomexchange.org/dataset/PXD001117).

Original languageEnglish
Pages (from-to)1332-1337
Number of pages6
JournalProteomics
Volume15
Issue number7
DOIs
Publication statusPublished - 1 Apr 2015

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Keywords

  • Biomedicine
  • Human cardiac stem cells
  • Nano-LC-MS/MS
  • Prefractionation
  • Receptome

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