Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells

Paulo J. da Costa, Juliane Menezes, Margarida Saramago, Juan F. García-Moreno, Hugo A. Santos, Margarida Gama-Carvalho, Cecília M. Arraiano, Sandra C. Viegas, Luísa Romão

Research output: Other contribution

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Abstract

In this article, we present supportive data related to the research article “A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells” [1], where interpretation of the data presented here is available. Indeed, here we analyze the impact of the DIS3L2 exoribonuclease over nonsense-mediated mRNA decay (NMD)-targets. Specifically, we present data on: a) the expression of various reporter human β-globin mRNAs, monitored by Northern blot and RT-qPCR, before and after altering DIS3L2 levels in HeLa cells, and b) the gene expression levels of deregulated transcripts generated by re-analyzing publicly available data from UPF1-depleted HeLa cells that were further cross-referenced with a dataset of transcripts upregulated in DIS3L2-depleted cells. These analyses revealed that DIS3L2 regulates the levels of a subset of NMD-targets. These data can be valuable for researchers interested in the NMD mechanism.

Original languageEnglish
Volume28
DOIs
Publication statusPublished - 1 Feb 2020

Publication series

NameData in brief
PublisherElsevier BV
ISSN (Print)2352-3409

Keywords

  • DIS3L2
  • mRNA degradation
  • mRNA surveillance
  • NMD
  • NMD-targets
  • UPF1

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