Experimental feeding of Sergentomyia minuta on reptiles and mammals: comparison with Phlebotomus papatasi

Lucie Ticha, Vera Volfova, Jairo Alfonso Mendoza-Roldan, Marcos Antonio Bezerra-Santos, Carla Maia, Jovana Sadlova, Domenico Otranto, Petr Volf

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5 Citations (Scopus)

Abstract

Background: Sergentomyiaminuta (Diptera: Phlebotominae) is an abundant sand fly species in the Mediterranean basin and a proven vector of reptile parasite Leishmania (Sauroleishmania) tarentolae. Although it feeds preferentially on reptiles, blood meal analyses and detection of Leishmania (Leishmania) infantum DNA in wild-caught S.minuta suggest that occasional feeding may occur on mammals, including humans. Therefore, it is currently suspected as a potential vector of human pathogens. Methods: A recently established S.minuta colony was allowed to feed on three reptile species (i.e. lizard Podarcissiculus and geckos Tarentolamauritanica and Hemidactylusturcicus) and three mammal species (i.e. mouse, rabbit and human). Sand fly mortality and fecundity were studied in blood-fed females, and the results were compared with Phlebotomuspapatasi, vector of Leishmania (L.) major. Blood meal volumes were measured by haemoglobinometry. Results: Sergentomyiaminuta fed readily on three reptile species tested, neglected the mouse and the rabbit but took a blood meal on human. However, the percentage of females engorged on human volunteer was low in cage (3%) and feeding on human blood resulted in extended defecation times, higher post-feeding mortality and lower fecundity. The average volumes of blood ingested by females fed on human and gecko were 0.97 µl and 1.02 µl, respectively. Phlebotomuspapatasi females readily fed on mouse, rabbit and human volunteer; a lower percentage of females (23%) took blood meal on the T.mauritanica gecko; reptilian blood increased mortality post-feeding but did not affect P.papatasi fecundity. Conclusions: Anthropophilic behaviour of S.minuta was experimentally demonstrated; although sand fly females prefer reptiles as hosts, they were attracted to the human volunteer and took a relatively high volume of blood. Their feeding times were longer than in sand fly species regularly feeding on mammals and their physiological parameters suggest that S.minuta is not adapted well for digestion of mammalian blood. Nevertheless, the ability to bite humans highlights the necessity of further studies on S.minuta vector competence to elucidate its potential role in circulation of Leishmania and phleboviruses pathogenic to humans. Graphical abstract: [Figure not available: see fulltext.].

Original languageEnglish
Article number126
JournalParasites and Vectors
Volume16
Issue number1
DOIs
Publication statusPublished - Dec 2023

Keywords

  • Feeding preferences
  • Leishmania
  • Phlebotomus
  • Sand flies
  • Sergentomyia

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