TY - JOUR
T1 - Exopolysaccharide production by the marine bacterium Alteromonas macleodii Mo169 using fruit pulp waste as the sole carbon source
AU - Concórdio-Reis, Patrícia
AU - Serafim, Bruno
AU - Pereira, João R.
AU - Moppert, Xavier
AU - Guézennec, Jean
AU - Reis, Maria A. M.
AU - Freitas, Filomena
N1 - Funding Information:
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT#
info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F131947%2F2017/PT#
info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F147518%2F2019/PT#
project LA/P/0140/202019 of the Associate Laboratory Institute for Health and Bioeconomy – i4HB.
Publisher Copyright:
© 2023 The Author(s)
PY - 2023/5
Y1 - 2023/5
N2 - A sugar-rich apple pulp waste generated from fruit processing for juice production was used as the sole carbon source for the cultivation of Alteromonas macleodii Mo169, a marine bacterium known for its EPS-secreting ability. The strain efficiently utilized the glucose and fructose present in the apple pulp waste, reaching biomass and EPS production of 9.20 ± 0.61 and 3.51 ± 0.08 g L−1, respectively, in 24-hour bioreactor cultivation. Two high molecular weight (Mw) fractions (1.7 ± 0.0 and 0.74 ± 0.0 MDa) were detected in the sample recovered from the cell-free supernatant by dialysis. The compositional analysis revealed the presence of glucose (31.1 ± 0.2 mol%), arabinose (23.9 ± 0.1 mol%), mannose (17.3 ± 0.1 mol%), glucosamine (10.3 ± 0.5 mol%), galactose (8.7 ± 0.0 mol%) and galacturonic acid (8.7 ± 0.0 mol%), as well as a high content in sulphate (6.0 ± 0.5 wt%). Given the presence of a high Mw polysaccharide in the apple pulp waste, probably pectin, a fraction of the detected sugar monomers might be attributed to that polymer, which was recovered together with A. macleodii Mo169 EPS. Concomitant with EPS synthesis, there was a viscosity build-up in the cultivation broth, which developed a shear-thinning fluid behaviour not observed in the initial medium. Therefore, this study demonstrates that apple pulp waste can be efficiently converted into a novel polysaccharide by A. macleodii Mo169 in a sustainable bioprocess. Moreover, the EPS sugar and acyl composition, together with its good thickening capacity, render the biopolymer of interest for use in several applications.
AB - A sugar-rich apple pulp waste generated from fruit processing for juice production was used as the sole carbon source for the cultivation of Alteromonas macleodii Mo169, a marine bacterium known for its EPS-secreting ability. The strain efficiently utilized the glucose and fructose present in the apple pulp waste, reaching biomass and EPS production of 9.20 ± 0.61 and 3.51 ± 0.08 g L−1, respectively, in 24-hour bioreactor cultivation. Two high molecular weight (Mw) fractions (1.7 ± 0.0 and 0.74 ± 0.0 MDa) were detected in the sample recovered from the cell-free supernatant by dialysis. The compositional analysis revealed the presence of glucose (31.1 ± 0.2 mol%), arabinose (23.9 ± 0.1 mol%), mannose (17.3 ± 0.1 mol%), glucosamine (10.3 ± 0.5 mol%), galactose (8.7 ± 0.0 mol%) and galacturonic acid (8.7 ± 0.0 mol%), as well as a high content in sulphate (6.0 ± 0.5 wt%). Given the presence of a high Mw polysaccharide in the apple pulp waste, probably pectin, a fraction of the detected sugar monomers might be attributed to that polymer, which was recovered together with A. macleodii Mo169 EPS. Concomitant with EPS synthesis, there was a viscosity build-up in the cultivation broth, which developed a shear-thinning fluid behaviour not observed in the initial medium. Therefore, this study demonstrates that apple pulp waste can be efficiently converted into a novel polysaccharide by A. macleodii Mo169 in a sustainable bioprocess. Moreover, the EPS sugar and acyl composition, together with its good thickening capacity, render the biopolymer of interest for use in several applications.
KW - Alteromonas macleodii Mo169
KW - Apple pulp waste
KW - Blue biotechnology
KW - Exopolysaccharide (EPS)
UR - http://www.scopus.com/inward/record.url?scp=85149439838&partnerID=8YFLogxK
U2 - 10.1016/j.eti.2023.103090
DO - 10.1016/j.eti.2023.103090
M3 - Article
AN - SCOPUS:85149439838
SN - 2352-1864
VL - 30
JO - Environmental Technology and Innovation
JF - Environmental Technology and Innovation
M1 - 103090
ER -