Exopolysaccharide production by a marine Pseudoalteromonas sp. strain isolated from Madeira Archipelago ocean sediments

Christophe Roca, Mareen Lehmann, Cristiana A V Torres, Sílvia Baptista, Susana P. Gaudêncio, Filomena Freitas, Maria A M Reis

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Exopolysaccharides (EPS) are polymers excreted by some microorganisms with interesting properties and used in many industrial applications. A new Pseudoalteromonas sp. strain, MD12-642, was isolated from marine sediments and cultivated in bioreactor in saline culture medium containing glucose as carbon source. Its ability to produce EPS under saline conditions was demonstrated reaching an EPS production of 4.4 g/L within 17 hours of cultivation, corresponding to a volumetric productivity of 0.25 g/L h, the highest value so far obtained for Pseudoalteromonas sp. strains. The compositional analysis of the EPS revealed the presence of galacturonic acid (41-42 mol%), glucuronic acid (25-26 mol%), rhamnose (16-22 mol%) and glucosamine (12-16 mol%) sugar residues. The polymer presents a high molecular weight (above 1000 kDa). These results encourage the biotechnological exploitation of strain MD12-642 for the production of valuable EPS with unique composition, using saline by-products/wastes as feedstocks.

Original languageEnglish
Pages (from-to)460-466
Number of pages7
JournalNew Biotechnology
Volume33
Issue number4
DOIs
Publication statusPublished - 25 Jun 2016

Keywords

  • SEA HYDROTHERMAL VENT
  • BACTERIAL EXOPOLYSACCHARIDES
  • ECOLOGICAL ROLES
  • POLYSACCHARIDE
  • FERMENTATION
  • MOLECULES
  • ACID

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