Evidence for selenocysteine coordination to the active site nickel in the [NiFeSe]hydrogenases from Desulfovibrio baculatus.

M. K. Eidsness, R. A. Scott, B. C. Prickril, D. V. DerVartanian, J. Legall, I. Moura, José J. G. Moura, H. D. Peck

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Abstract

Ni and Se x-ray absorption spectroscopic studies of the [NiFeSe]hydrogenases from Desulfovibrio baculatus are described. The Ni site geometry is pseudo-octahedral with a coordinating ligand composition of 3-4 (N,O) at 2.06 A, 1-2 (S,Cl) at 2.17 A, and 1 Se at 2.44 A. The Se coordination environment consists of 1 C at 2.0 A and a heavy scatterer M (M = Ni or Fe) at approximately 2.4 A. These results are interpreted in terms of a selenocysteine residue coordinated to the Ni site. The possible role of the Ni-Se site in the catalytic activation of H2 is discussed.

Original languageEnglish
Pages (from-to)147-151
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number1
DOIs
Publication statusPublished - 1 Jan 1989

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