Evening and morning peroxiredoxin-2 redox/oligomeric state changes in obstructive sleep apnea red blood cells

Correlation with polysomnographic and metabolic parameters

Amélia Feliciano, Fátima Vaz, Vukosava M Torres, Cristina Valentim-Coelho, Rita Silva, Vesna Prosinecki, Bruno M Alexandre, Ana Sofia Carvalho, Rune Matthiesen, Atul Malhotra, Paula Pinto, Cristina Bárbara, Deborah Penque

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have examined the effects of Obstructive Sleep Apnea (OSA) on red blood cell (RBC) proteome variation at evening/morning day time to uncover new insights into OSA-induced RBC dysfunction that may lead to OSA manifestations. Dysregulated proteins mainly fall in the group of catalytic enzymes, stress response and redox regulators such as peroxiredoxin 2 (PRDX2). Validation assays confirmed that at morning the monomeric/dimeric forms of PRDX2 were more overoxidized in OSA RBC compared to evening samples. Six month of positive airway pressure (PAP) treatment decreased this overoxidation and generated multimeric overoxidized forms associated with chaperone/transduction signaling activity of PRDX2. Morning levels of overoxidized PRDX2 correlated with polysomnographic (PSG)-arousal index and metabolic parameters whereas the evening level of disulfide-linked dimer (associated with peroxidase activity of PRDX2) correlated with PSG parameters. After treatment, morning overoxidized multimer of PRDX2 negatively correlated with fasting glucose and dopamine levels. Overall, these data point toward severe oxidative stress and altered antioxidant homeostasis in OSA RBC occurring mainly at morning time but with consequences till evening. The beneficial effect of PAP involves modulation of the redox/oligomeric state of PRDX2, whose mechanism and associated chaperone/transduction signaling functions deserves further investigation. RBC PRDX2 is a promising candidate biomarker for OSA severity and treatment monitoring, warranting further investigation and validation.

Original languageEnglish
Pages (from-to)621-629
Number of pages9
JournalBiochimica Et Biophysica Acta
Volume1863
Issue number2
DOIs
Publication statusPublished - Feb 2017

Fingerprint

Peroxiredoxins
Obstructive Sleep Apnea
Oxidation-Reduction
Erythrocytes
Pressure
Proteome
Arousal
Disulfides
Peroxidase
Fasting
Dopamine
Oxidative Stress
Homeostasis
Therapeutics
Antioxidants
Biomarkers
Glucose

Keywords

  • Biomarkers
  • Obstructive sleep apnea
  • Peroxiredeoxin-2
  • Red blood cells

Cite this

Feliciano, Amélia ; Vaz, Fátima ; Torres, Vukosava M ; Valentim-Coelho, Cristina ; Silva, Rita ; Prosinecki, Vesna ; Alexandre, Bruno M ; Carvalho, Ana Sofia ; Matthiesen, Rune ; Malhotra, Atul ; Pinto, Paula ; Bárbara, Cristina ; Penque, Deborah. / Evening and morning peroxiredoxin-2 redox/oligomeric state changes in obstructive sleep apnea red blood cells : Correlation with polysomnographic and metabolic parameters. In: Biochimica Et Biophysica Acta. 2017 ; Vol. 1863, No. 2. pp. 621-629.
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abstract = "We have examined the effects of Obstructive Sleep Apnea (OSA) on red blood cell (RBC) proteome variation at evening/morning day time to uncover new insights into OSA-induced RBC dysfunction that may lead to OSA manifestations. Dysregulated proteins mainly fall in the group of catalytic enzymes, stress response and redox regulators such as peroxiredoxin 2 (PRDX2). Validation assays confirmed that at morning the monomeric/dimeric forms of PRDX2 were more overoxidized in OSA RBC compared to evening samples. Six month of positive airway pressure (PAP) treatment decreased this overoxidation and generated multimeric overoxidized forms associated with chaperone/transduction signaling activity of PRDX2. Morning levels of overoxidized PRDX2 correlated with polysomnographic (PSG)-arousal index and metabolic parameters whereas the evening level of disulfide-linked dimer (associated with peroxidase activity of PRDX2) correlated with PSG parameters. After treatment, morning overoxidized multimer of PRDX2 negatively correlated with fasting glucose and dopamine levels. Overall, these data point toward severe oxidative stress and altered antioxidant homeostasis in OSA RBC occurring mainly at morning time but with consequences till evening. The beneficial effect of PAP involves modulation of the redox/oligomeric state of PRDX2, whose mechanism and associated chaperone/transduction signaling functions deserves further investigation. RBC PRDX2 is a promising candidate biomarker for OSA severity and treatment monitoring, warranting further investigation and validation.",
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Feliciano, A, Vaz, F, Torres, VM, Valentim-Coelho, C, Silva, R, Prosinecki, V, Alexandre, BM, Carvalho, AS, Matthiesen, R, Malhotra, A, Pinto, P, Bárbara, C & Penque, D 2017, 'Evening and morning peroxiredoxin-2 redox/oligomeric state changes in obstructive sleep apnea red blood cells: Correlation with polysomnographic and metabolic parameters', Biochimica Et Biophysica Acta, vol. 1863, no. 2, pp. 621-629. https://doi.org/10.1016/j.bbadis.2016.11.019

Evening and morning peroxiredoxin-2 redox/oligomeric state changes in obstructive sleep apnea red blood cells : Correlation with polysomnographic and metabolic parameters. / Feliciano, Amélia; Vaz, Fátima; Torres, Vukosava M; Valentim-Coelho, Cristina; Silva, Rita; Prosinecki, Vesna; Alexandre, Bruno M; Carvalho, Ana Sofia; Matthiesen, Rune; Malhotra, Atul; Pinto, Paula; Bárbara, Cristina; Penque, Deborah.

In: Biochimica Et Biophysica Acta, Vol. 1863, No. 2, 02.2017, p. 621-629.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evening and morning peroxiredoxin-2 redox/oligomeric state changes in obstructive sleep apnea red blood cells

T2 - Correlation with polysomnographic and metabolic parameters

AU - Feliciano, Amélia

AU - Vaz, Fátima

AU - Torres, Vukosava M

AU - Valentim-Coelho, Cristina

AU - Silva, Rita

AU - Prosinecki, Vesna

AU - Alexandre, Bruno M

AU - Carvalho, Ana Sofia

AU - Matthiesen, Rune

AU - Malhotra, Atul

AU - Pinto, Paula

AU - Bárbara, Cristina

AU - Penque, Deborah

N1 - Copyright © 2016. Published by Elsevier B.V.

PY - 2017/2

Y1 - 2017/2

N2 - We have examined the effects of Obstructive Sleep Apnea (OSA) on red blood cell (RBC) proteome variation at evening/morning day time to uncover new insights into OSA-induced RBC dysfunction that may lead to OSA manifestations. Dysregulated proteins mainly fall in the group of catalytic enzymes, stress response and redox regulators such as peroxiredoxin 2 (PRDX2). Validation assays confirmed that at morning the monomeric/dimeric forms of PRDX2 were more overoxidized in OSA RBC compared to evening samples. Six month of positive airway pressure (PAP) treatment decreased this overoxidation and generated multimeric overoxidized forms associated with chaperone/transduction signaling activity of PRDX2. Morning levels of overoxidized PRDX2 correlated with polysomnographic (PSG)-arousal index and metabolic parameters whereas the evening level of disulfide-linked dimer (associated with peroxidase activity of PRDX2) correlated with PSG parameters. After treatment, morning overoxidized multimer of PRDX2 negatively correlated with fasting glucose and dopamine levels. Overall, these data point toward severe oxidative stress and altered antioxidant homeostasis in OSA RBC occurring mainly at morning time but with consequences till evening. The beneficial effect of PAP involves modulation of the redox/oligomeric state of PRDX2, whose mechanism and associated chaperone/transduction signaling functions deserves further investigation. RBC PRDX2 is a promising candidate biomarker for OSA severity and treatment monitoring, warranting further investigation and validation.

AB - We have examined the effects of Obstructive Sleep Apnea (OSA) on red blood cell (RBC) proteome variation at evening/morning day time to uncover new insights into OSA-induced RBC dysfunction that may lead to OSA manifestations. Dysregulated proteins mainly fall in the group of catalytic enzymes, stress response and redox regulators such as peroxiredoxin 2 (PRDX2). Validation assays confirmed that at morning the monomeric/dimeric forms of PRDX2 were more overoxidized in OSA RBC compared to evening samples. Six month of positive airway pressure (PAP) treatment decreased this overoxidation and generated multimeric overoxidized forms associated with chaperone/transduction signaling activity of PRDX2. Morning levels of overoxidized PRDX2 correlated with polysomnographic (PSG)-arousal index and metabolic parameters whereas the evening level of disulfide-linked dimer (associated with peroxidase activity of PRDX2) correlated with PSG parameters. After treatment, morning overoxidized multimer of PRDX2 negatively correlated with fasting glucose and dopamine levels. Overall, these data point toward severe oxidative stress and altered antioxidant homeostasis in OSA RBC occurring mainly at morning time but with consequences till evening. The beneficial effect of PAP involves modulation of the redox/oligomeric state of PRDX2, whose mechanism and associated chaperone/transduction signaling functions deserves further investigation. RBC PRDX2 is a promising candidate biomarker for OSA severity and treatment monitoring, warranting further investigation and validation.

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