TY - JOUR
T1 - Evaluation of the hydroalcoholic extract of Clarisia racemosa as an antiparasitic agent
T2 - an in vitro approach
AU - da Cruz Filho, Iranildo José
AU - Duarte, Denise Maria Figueiredo Araújo
AU - Marques, Diego Santa Clara
AU - da Rocha, João Victor Ritinto
AU - Diniz, Emily Gabriele Marques
AU - Brayner, Fábio André
AU - Alves, Luiz Carlos
AU - de Azevedo Albuquerque, Mônica Camelo Pessoa
AU - de Lima Aires, André
AU - Nogueira, Fátima
AU - de Lima, Maria do Carmo Alves
N1 - Funding Information:
The authors thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for a grant and M.C.A. Lima Fellowship (Process 306865/612020-3); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES—Finance Code N° 001) and the Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE). J.V.R. Rocha and I.J. Cruz Filho would like to thank FACEPE for the Graduate Scholarship (Process PBPG-1832-4.01/22) and Researcher Fixation Scholarships (Process BFP-0038-4.03/21), respectively. In addition, M.C.A. Lima and A.L. Aires would like to thank FACEPE Research Project Aid (Process APQ-0498-4.03/19) and (Process APQ-Emergent 1181-4.03/22), respectively. We would like to thank Mil Madeiras Preciosas, a subsidiary of the Swiss group Precious Woods ( http://preciouswoods.com.br/ ) for supplying the Clarisia racemosa trunks. Thanks to MR4, who provided us with the Plasmodium falciparum MRA-1029 strain provided by Andrew Talman, Robert Sinden that we used in the trials. The work was partially supported by the FCT project reference CIRCNA/BRB/0281/2019_AMAZING and GHTM-UID/Multi/04413/2013.
Funding Information:
The authors thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for a grant and M.C.A. Lima Fellowship (Process 306865/612020-3); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES—Finance Code N° 001) and the Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE). J.V.R. Rocha and I.J. Cruz Filho would like to thank FACEPE for the Graduate Scholarship (Process PBPG-1832-4.01/22) and Researcher Fixation Scholarships (Process BFP-0038-4.03/21), respectively. In addition, M.C.A. Lima and A.L. Aires would like to thank FACEPE Research Project Aid (Process APQ-0498-4.03/19) and (Process APQ-Emergent 1181-4.03/22), respectively. We would like to thank Mil Madeiras Preciosas, a subsidiary of the Swiss group Precious Woods (http://preciouswoods.com.br/) for supplying the Clarisia racemosa trunks. Thanks to MR4, who provided us with the Plasmodium falciparum MRA-1029 strain provided by Andrew Talman, Robert Sinden that we used in the trials. The work was partially supported by the FCT project reference CIRCNA/BRB/0281/2019_AMAZING and GHTM-UID/Multi/04413/2013.
Publisher Copyright:
© 2023, King Abdulaziz City for Science and Technology.
PY - 2023/12
Y1 - 2023/12
N2 - Clarisia racemosa Ruiz & Pav is a neotropical species found in humid forests from southern Mexico to southern Brazil. There are few studies related to the ethnopharmacological use of C. racemosa. Our objective was to evaluate the hydroalcoholic extract of C. racemosa as a potential antiparasitic agent. For this, we performed in vitro assays against strains of Leishmania amazonensis, Trypanosoma cruzi, Plasmodium falciparum, and Schistosoma mansoni. At the same time, immunomodulatory activity tests were carried out. The results demonstrated that the extract was able to stimulate and activate immune cells. In preliminary antiparasitic tests, structural modifications were observed in the promastigote form of L. amazonensis and in adult worms of S. mansoni. The extract was able to inhibit the growth of trypomastigote form of T. cruzi and finally showed low antiparasitic activity against strains of P. falciparum. It is pioneering work and these results demonstrate that C. racemosa extract is a promising alternative and contributes to the arsenal of possible forms of treatment to combat parasites.
AB - Clarisia racemosa Ruiz & Pav is a neotropical species found in humid forests from southern Mexico to southern Brazil. There are few studies related to the ethnopharmacological use of C. racemosa. Our objective was to evaluate the hydroalcoholic extract of C. racemosa as a potential antiparasitic agent. For this, we performed in vitro assays against strains of Leishmania amazonensis, Trypanosoma cruzi, Plasmodium falciparum, and Schistosoma mansoni. At the same time, immunomodulatory activity tests were carried out. The results demonstrated that the extract was able to stimulate and activate immune cells. In preliminary antiparasitic tests, structural modifications were observed in the promastigote form of L. amazonensis and in adult worms of S. mansoni. The extract was able to inhibit the growth of trypomastigote form of T. cruzi and finally showed low antiparasitic activity against strains of P. falciparum. It is pioneering work and these results demonstrate that C. racemosa extract is a promising alternative and contributes to the arsenal of possible forms of treatment to combat parasites.
KW - Amazon forest
KW - Antiparasitic agent
KW - Clarisia racemosa
KW - Natural products
UR - http://www.scopus.com/inward/record.url?scp=85176104468&partnerID=8YFLogxK
U2 - 10.1007/s13205-023-03799-2
DO - 10.1007/s13205-023-03799-2
M3 - Article
C2 - 37953832
AN - SCOPUS:85176104468
SN - 2190-572X
VL - 13
JO - 3 Biotech
JF - 3 Biotech
IS - 12
M1 - 391
ER -
