Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles

A P C Ribeiro; S Anbu; E C B A Alegria; A R Fernandes; P V Baptista; R Mendes; A S Matias; M Mendes; M F C Guedes da Silva; A J L Pombeiro

doi:10.1016/j.biopha.2018.02.069

Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles

A P C Ribeiro, S Anbu, E C B A Alegria, A R Fernandes, P V Baptista, R Mendes, A S Matias, M Mendes, M F C Guedes da Silva, A J L Pombeiro

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 ± 0.1 μM) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 ± 0.1 μM). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.

Original language	English
Pages (from-to)	137-144
Number of pages	8
Journal	Biomedicine and Pharmacotherapy
Volume	101
DOIs	https://doi.org/10.1016/j.biopha.2018.02.069
Publication status	Published - May 2018

Keywords

Green chemistry
Silver nanoparticles (AgNPs)
DNA interaction
Cytotoxicity
Cancer cell line
BSA interaction

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biopha.2018.02.069Licence: CC BY-NC-ND

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Ribeiro, A. P. C., Anbu, S., Alegria, E. C. B. A., Fernandes, A. R., Baptista, P. V., Mendes, R., Matias, A. S., Mendes, M., Guedes da Silva, M. F. C., & Pombeiro, A. J. L. (2018). Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles. Biomedicine and Pharmacotherapy, 101, 137-144. https://doi.org/10.1016/j.biopha.2018.02.069

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abstract = "Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 ± 0.1 μM) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 ± 0.1 μM). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.",

keywords = "Green chemistry, Silver nanoparticles (AgNPs), DNA interaction, Cytotoxicity, Cancer cell line, BSA interaction",

author = "Ribeiro, {A P C} and S Anbu and Alegria, {E C B A} and Fernandes, {A R} and Baptista, {P V} and R Mendes and Matias, {A S} and M Mendes and {Guedes da Silva}, {M F C} and Pombeiro, {A J L}",

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AU - Ribeiro, A P C

AU - Anbu, S

AU - Alegria, E C B A

AU - Fernandes, A R

AU - Baptista, P V

AU - Mendes, R

AU - Matias, A S

AU - Mendes, M

AU - Guedes da Silva, M F C

AU - Pombeiro, A J L

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N2 - Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 ± 0.1 μM) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 ± 0.1 μM). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.

AB - Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 ± 0.1 μM) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 ± 0.1 μM). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.

KW - Green chemistry

KW - Silver nanoparticles (AgNPs)

KW - DNA interaction

KW - Cytotoxicity

KW - Cancer cell line

KW - BSA interaction

U2 - 10.1016/j.biopha.2018.02.069

DO - 10.1016/j.biopha.2018.02.069

M3 - Article

C2 - 29482059

SN - 0753-3322

VL - 101

SP - 137

EP - 144

JO - Biomedicine and Pharmacotherapy

JF - Biomedicine and Pharmacotherapy

ER -

Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles

Abstract

Keywords

UN SDGs

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