Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles

A P C Ribeiro, S Anbu, E C B A Alegria, A R Fernandes, P V Baptista, R Mendes, A S Matias, M Mendes, M F C Guedes da Silva, A J L Pombeiro

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Silver nanoparticles (AgNPs) were prepared by GREEN chemistry relying on the reduction of AgNO3 by phytochemicals present in black tea extract. AgNPs were fully characterized by transmission electron microscopy (TEM), ultraviolet-visible spectroscopy ((UV-vis)), X-ray diffraction (XRD) and energy dispersive absorption spectroscopy (EDS). The synthesized AgNPs induced a decrease of the cell viability in a dose-dependent manner with a low IC50 (0.5 ± 0.1 μM) for an ovarian carcinoma cell line (A2780) compared to primary human fibroblasts (IC50 5.0 ± 0.1 μM). The DNA binding capability of CT (calf thymus) DNA was investigated using electronic absorption and fluorescence spectroscopies, circular dichroism and viscosity titration methods. Additionally, the AgNPs strongly quench the intrinsic fluorescence of BSA, as determined by synchronous fluorescence spectra.

Original languageEnglish
Pages (from-to)137-144
Number of pages8
JournalBiomedicine and Pharmacotherapy
Volume101
DOIs
Publication statusPublished - May 2018

Keywords

  • Green chemistry
  • Silver nanoparticles (AgNPs)
  • DNA interaction
  • Cytotoxicity
  • Cancer cell line
  • BSA interaction

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