@inbook{6a1a2ff21fa04e12aa9ebb73398c85a5,
title = "Evaluating Root Mechanosensing Response in Rice",
abstract = "Unable to move, plants are physically restrained to the place where they grow. Remarkably, plants have developed a myriad of mechanisms to perceive the surrounding environment in order to maximize growth and survival. One of those mechanisms is the ability to perceive mechanical stimulus such as touch (thigmomorphogenesis), in order to adjust growth patterns (in different organs) to either attach to or surround an object. Roots are able to perceive several mechanical forces (e.g., gravity, touch). However, being the “hidden part” of a plant, it is difficult to assess their response to mechanical stimulation. In this chapter, our team presents a simple method to evaluate rice (Oryza sativa L.) root mechanosensing response that can be used to test different conditions (e.g., hormones) affecting rice root response to touch stimulus. This method is affordable to any lab and can be upgraded with a fully automated image recording system. We provide a detailed protocol with several notes for a more comprehensive application.",
keywords = "Coiling, Curling, Helicoidal, Thigmomorphogenesis, Touch, “Sandwich” method",
author = "Louren{\c c}o, {Tiago F.} and Cordeiro, {Andr{\'e} M.} and Jo{\~a}o Fraz{\~a}o and Saibo, {Nelson J.M.} and Oliveira, {M. Margarida}",
note = "Funding Information: The authors would like to acknowledge the Champalimaud Foundation (Hardware Platform) for the support in the imaging setup and Fredilson Melo for the support in the preparation of the figures. This work was supported by Funda??o para a Ci?ncia e a Tecnologia (FCT) through an Assistant Researcher contract to TFL (CEE-CIND/03641/2017), a postdoc contract to AMC within FCT Project PTDC/BIA-FBT/31070/2017. Funding from the Research Unit ?GREEN-IT ? Bioresources for Sustainability? (UIDB/04551/2020 and UIDP/04551/2020) and the FCT Research Grant PTDC/BIA-FBT/31070/2017 is also acknowledged. Funding Information: The authors would like to acknowledge the Champalimaud Foundation (Hardware Platform) for the support in the imaging setup and Fredilson Melo for the support in the preparation of the figures. This work was supported by Fundac¸{\~a}o para a Ci{\^e}ncia e a Tecnolo-gia (FCT) through an Assistant Researcher contract to TFL (CEE-CIND/03641/2017), a postdoc contract to AMC within FCT Project PTDC/BIA-FBT/31070/2017. Funding from the Research Unit “GREEN-IT – Bioresources for Sustainability” (UIDB/04551/2020 and UIDP/04551/2020) and the FCT Research Grant PTDC/BIA-FBT/31070/2017 is also acknowledged. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2297-1_3",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "25--35",
booktitle = "Methods in Molecular Biology",
address = "United States",
}