Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via β-alanine

Irina Borodina, Kanchana R. Kildegaard, Niels B. Jensen, Thomas H. Blicher, JérÔme Maury, Svetlana Sherstyk, Konstantin Schneider, Pedro Lamosa, Markus J. Herrgård, Inger Rosenstand, Fredrik Öberg, Jochen Forster, Jens Nielsen

Research output: Contribution to journalArticlepeer-review

112 Citations (Scopus)

Abstract

Microbial fermentation of renewable feedstocks into plastic monomers can decrease our fossil dependence and reduce global CO2 emissions. 3-Hydroxypropionic acid (3HP) is a potential chemical building block for sustainable production of superabsorbent polymers and acrylic plastics. With the objective of developing Saccharomyces cerevisiae as an efficient cell factory for high-level production of 3HP, we identified the β-alanine biosynthetic route as the most economically attractive according to the metabolic modeling. We engineered and optimized a synthetic pathway for de novo biosynthesis of β-alanine and its subsequent conversion into 3HP using a novel β-alanine-pyruvate aminotransferase discovered in Bacillus cereus. The final strain produced 3HP at a titer of 13.7±0.3gL-1 with a 0.14±0.0C-molC-mol-1 yield on glucose in 80h in controlled fed-batch fermentation in mineral medium at pH 5, and this work therefore lays the basis for developing a process for biological 3HP production.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalMetabolic Engineering
Volume27
DOIs
Publication statusPublished - 1 Jan 2015

Keywords

  • 3-hydroxypropionic acid
  • Biosustainable acrylics
  • Saccharomyces cerevisiae
  • β-alanine
  • β-alanine-pyruvate aminotransferase

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