TY - JOUR
T1 - EPR spectroscopy of protein microcrystals oriented in a liquid crystalline polymer medium
AU - Caldeira, J.
AU - Figueirinhas, João Luís Maia
AU - Santos, Celina
AU - Godinho, Maria Helena Figueiredo
PY - 2004/10
Y1 - 2004/10
N2 - Correlation of the g-tensor of a paramagnetic active center of a protein with its structure provides a unique experimental information on the electronic structure of the metal site. To address this problem, we made solid films containing metalloprotein (Desulfovibrio gigas cytochrome c 3) microcrystals. The microcrystals in a liquid crystalline polymer medium (water/hydroxypropylcellulose) were partially aligned by a shear flow. A strong orientation effect of the metalloprotein was observed by EPR spectroscopy and polarizing optical microscopy. The EPR spectra of partially oriented samples were simulated, allowing for molecular orientation distribution function determination. The observed effect results in enhanced sensitivity and resolution of the EPR spectra and provides a new approach towards the correlation of spectroscopic data, obtained by EPR or some other technique, with the three-dimensional structure of a protein or a model compound.
AB - Correlation of the g-tensor of a paramagnetic active center of a protein with its structure provides a unique experimental information on the electronic structure of the metal site. To address this problem, we made solid films containing metalloprotein (Desulfovibrio gigas cytochrome c 3) microcrystals. The microcrystals in a liquid crystalline polymer medium (water/hydroxypropylcellulose) were partially aligned by a shear flow. A strong orientation effect of the metalloprotein was observed by EPR spectroscopy and polarizing optical microscopy. The EPR spectra of partially oriented samples were simulated, allowing for molecular orientation distribution function determination. The observed effect results in enhanced sensitivity and resolution of the EPR spectra and provides a new approach towards the correlation of spectroscopic data, obtained by EPR or some other technique, with the three-dimensional structure of a protein or a model compound.
KW - Metalloproteins
KW - Orientation
KW - EPR
KW - Liquid crystalline polymers
UR - http://www.scopus.com/record/display.uri?eid=2-s2.0-4544261377&origin=inward&txGid=c8e142320cae4fc57218f063dcda3621#
U2 - 10.1016/j.jmr.2004.07.003
DO - 10.1016/j.jmr.2004.07.003
M3 - Article
VL - 170
SP - 213
EP - 219
JO - Journal Of Magnetic Resonance
JF - Journal Of Magnetic Resonance
SN - 1090-7807
IS - 2
ER -