Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells

Eva Susnik, Amelie Bazzoni, Patricia Taladriz-Blanco, Sandor Balog, Aura Maria Moreno-Echeverri, Christina Glaubitz, Beatriz Brito Oliveira, Daniela Ferreira, Pedro Viana Baptista, Alke Petri-Fink, Barbara Rothen-Rutishauser

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
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Abstract

Introduction: Delivery of therapeutic nanoparticles (NPs) to cancer cells represents a promising approach for biomedical applications. A key challenge for nanotechnology translation from the bench to the bedside is the low amount of administered NPs dose that effectively enters target cells. To improve NPs delivery, several studies proposed NPs conjugation with ligands, which specifically deliver NPs to target cells via receptor binding. One such example is epidermal growth factor (EGF), a peptide involved in cell signaling pathways that control cell division by binding to epidermal growth factor receptor (EGFR). However, very few studies assessed the influence of EGF present in the cell environment, on the cellular uptake of NPs. Methods: We tested if the stimulation of EGFR-expressing lung carcinomacells A549 with EGF affects the uptake of 59 nm and 422 nm silica (SiO2) NPs. Additionally, we investigated whether the uptake enhancement can be achieved with gold NPs, suitable to downregulate the expression of cancer oncogene c-MYC. Results: Our findings show that EGF binding to its receptor results in receptor autophosphorylation and initiate signaling pathways, leading to enhanced endocytosis of 59 nm SiO2 NPs, but not 422 nm SiO2 NPs. Additionally, we demonstrated an enhanced gold (Au) NPs endocytosis and subsequently a higher downregulation of c-MYC. Discussion: These findings contribute to a better understanding of NPs uptake in the presence of EGF and that is a promising approach for improved NPs delivery.
Original languageEnglish
Article number1220514
Number of pages17
JournalFrontiers in Nanotechnology
Volume5
DOIs
Publication statusPublished - 17 Jul 2023

Keywords

  • endocytosis
  • epidermal growth factor
  • gene silencing
  • gold
  • molecular mechanisms
  • nanomedicine
  • nanoparticles
  • silica

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