Epidemiological and genetic studies suggest a common Leishmania infantum transmission cycle in wildlife, dogs and humans associated to vector abundance in Southeast Spain

J Risueño, Ortuño, María, P. Pérez-Cutillas, E. Goyena, C Maia, S Cortes, L Campino, L. J. Bernal, Muñoz, Clara, I. Arcenillas, F. J. Martínez-Rondán, M. Gonzálvez, F. Collantes, Ortíz, Juana M., C. Martínez-Carrasco, E. Berriatua

Research output: Contribution to journalArticle

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Abstract

Leishmania infantum infection was investigated in 202 wild carnivores, rodents and lagomorphs in Southeast Spain using a real-time PCR (rtPCR) in skin and organ samples, mostly spleen. Lesions compatible with leishmaniosis were not observed in any of the animals. Prevalence defined as the percentage of rtPCR-positive animals was 32% overall, and 45% in foxes (n = 69), 30% in rabbits (n = 80) and stone martens (n = 10), 19% in wood mice (n = 16), 0% in black rats (n = 10) and ranged between 0% and 100% in other minoritarian species including badgers, wild cats, wolves, raccoons, genets and hares. Most infected rabbits were rtPCR-positive in skin and not in spleen samples and the opposite was the case for foxes (p < 0.05). L. infantum prevalence was lowest in spring following months of non-exposure to phlebotomine sand fly vectors, and spatially matched recently estimated Phlebotomus perniciosus vector abundance and the prevalence of subclinical infection in dogs and humans. Prevalence increased with altitude and was greater in drier and less windy South and West compared to the coastal Southeast of the study area (p < 0.05). Genetic diversity of L. infantum from foxes, investigated by PCR-restriction fragment length polymorphisms of kinetoplast DNA, revealed B genotype in all animals, which is frequent in people and dogs in the Iberian Peninsula and Morocco. The study provides further evidence that subclinical L. infantum infection is widespread in wildlife with prevalence depending on environmental factors and that parasite tissue tropism may vary according to host species. Moreover, it suggests that sylvatic and domestic transmission cycles are closely interconnected.
Original languageEnglish
Pages (from-to)61-67
Number of pages7
JournalVeterinary Parasitology
VolumeVol. 259
DOIs
Publication statusPublished - 15 Aug 2018

Fingerprint

Leishmania infantum
Spain
Epidemiologic Studies
wildlife
Dogs
foxes
dogs
Mustelidae
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
Phlebotominae
skin (animal)
spleen
Spleen
Phlebotomus perniciosus
Lagomorpha
rabbits
Kinetoplast DNA
Raccoons
infection

Keywords

  • Distribution
  • Genotypes
  • Leishmania infantum
  • PCR diagnosis
  • Wildlife
  • Spain

Cite this

Risueño, J ; María, Ortuño, ; Pérez-Cutillas, P. ; Goyena, E. ; Maia, C ; Cortes, S ; Campino, L ; Bernal, L. J. ; Clara, Muñoz, ; Arcenillas, I. ; Martínez-Rondán, F. J. ; Gonzálvez, M. ; Collantes, F. ; Juana M., Ortíz, ; Martínez-Carrasco, C. ; Berriatua, E. / Epidemiological and genetic studies suggest a common Leishmania infantum transmission cycle in wildlife, dogs and humans associated to vector abundance in Southeast Spain. In: Veterinary Parasitology. 2018 ; Vol. Vol. 259. pp. 61-67.
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abstract = "Leishmania infantum infection was investigated in 202 wild carnivores, rodents and lagomorphs in Southeast Spain using a real-time PCR (rtPCR) in skin and organ samples, mostly spleen. Lesions compatible with leishmaniosis were not observed in any of the animals. Prevalence defined as the percentage of rtPCR-positive animals was 32{\%} overall, and 45{\%} in foxes (n = 69), 30{\%} in rabbits (n = 80) and stone martens (n = 10), 19{\%} in wood mice (n = 16), 0{\%} in black rats (n = 10) and ranged between 0{\%} and 100{\%} in other minoritarian species including badgers, wild cats, wolves, raccoons, genets and hares. Most infected rabbits were rtPCR-positive in skin and not in spleen samples and the opposite was the case for foxes (p < 0.05). L. infantum prevalence was lowest in spring following months of non-exposure to phlebotomine sand fly vectors, and spatially matched recently estimated Phlebotomus perniciosus vector abundance and the prevalence of subclinical infection in dogs and humans. Prevalence increased with altitude and was greater in drier and less windy South and West compared to the coastal Southeast of the study area (p < 0.05). Genetic diversity of L. infantum from foxes, investigated by PCR-restriction fragment length polymorphisms of kinetoplast DNA, revealed B genotype in all animals, which is frequent in people and dogs in the Iberian Peninsula and Morocco. The study provides further evidence that subclinical L. infantum infection is widespread in wildlife with prevalence depending on environmental factors and that parasite tissue tropism may vary according to host species. Moreover, it suggests that sylvatic and domestic transmission cycles are closely interconnected.",
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Risueño, J, María, O, Pérez-Cutillas, P, Goyena, E, Maia, C, Cortes, S, Campino, L, Bernal, LJ, Clara, M, Arcenillas, I, Martínez-Rondán, FJ, Gonzálvez, M, Collantes, F, Juana M., O, Martínez-Carrasco, C & Berriatua, E 2018, 'Epidemiological and genetic studies suggest a common Leishmania infantum transmission cycle in wildlife, dogs and humans associated to vector abundance in Southeast Spain', Veterinary Parasitology, vol. Vol. 259, pp. 61-67. https://doi.org/10.1016/j.vetpar.2018.05.012

Epidemiological and genetic studies suggest a common Leishmania infantum transmission cycle in wildlife, dogs and humans associated to vector abundance in Southeast Spain. / Risueño, J; María, Ortuño,; Pérez-Cutillas, P.; Goyena, E.; Maia, C; Cortes, S; Campino, L; Bernal, L. J.; Clara, Muñoz, ; Arcenillas, I.; Martínez-Rondán, F. J.; Gonzálvez, M.; Collantes, F.; Juana M., Ortíz, ; Martínez-Carrasco, C.; Berriatua, E.

In: Veterinary Parasitology, Vol. Vol. 259, 15.08.2018, p. 61-67.

Research output: Contribution to journalArticle

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T1 - Epidemiological and genetic studies suggest a common Leishmania infantum transmission cycle in wildlife, dogs and humans associated to vector abundance in Southeast Spain

AU - Risueño, J

AU - María, Ortuño,

AU - Pérez-Cutillas, P.

AU - Goyena, E.

AU - Maia, C

AU - Cortes, S

AU - Campino, L

AU - Bernal, L. J.

AU - Clara, Muñoz,

AU - Arcenillas, I.

AU - Martínez-Rondán, F. J.

AU - Gonzálvez, M.

AU - Collantes, F.

AU - Juana M., Ortíz,

AU - Martínez-Carrasco, C.

AU - Berriatua, E.

PY - 2018/8/15

Y1 - 2018/8/15

N2 - Leishmania infantum infection was investigated in 202 wild carnivores, rodents and lagomorphs in Southeast Spain using a real-time PCR (rtPCR) in skin and organ samples, mostly spleen. Lesions compatible with leishmaniosis were not observed in any of the animals. Prevalence defined as the percentage of rtPCR-positive animals was 32% overall, and 45% in foxes (n = 69), 30% in rabbits (n = 80) and stone martens (n = 10), 19% in wood mice (n = 16), 0% in black rats (n = 10) and ranged between 0% and 100% in other minoritarian species including badgers, wild cats, wolves, raccoons, genets and hares. Most infected rabbits were rtPCR-positive in skin and not in spleen samples and the opposite was the case for foxes (p < 0.05). L. infantum prevalence was lowest in spring following months of non-exposure to phlebotomine sand fly vectors, and spatially matched recently estimated Phlebotomus perniciosus vector abundance and the prevalence of subclinical infection in dogs and humans. Prevalence increased with altitude and was greater in drier and less windy South and West compared to the coastal Southeast of the study area (p < 0.05). Genetic diversity of L. infantum from foxes, investigated by PCR-restriction fragment length polymorphisms of kinetoplast DNA, revealed B genotype in all animals, which is frequent in people and dogs in the Iberian Peninsula and Morocco. The study provides further evidence that subclinical L. infantum infection is widespread in wildlife with prevalence depending on environmental factors and that parasite tissue tropism may vary according to host species. Moreover, it suggests that sylvatic and domestic transmission cycles are closely interconnected.

AB - Leishmania infantum infection was investigated in 202 wild carnivores, rodents and lagomorphs in Southeast Spain using a real-time PCR (rtPCR) in skin and organ samples, mostly spleen. Lesions compatible with leishmaniosis were not observed in any of the animals. Prevalence defined as the percentage of rtPCR-positive animals was 32% overall, and 45% in foxes (n = 69), 30% in rabbits (n = 80) and stone martens (n = 10), 19% in wood mice (n = 16), 0% in black rats (n = 10) and ranged between 0% and 100% in other minoritarian species including badgers, wild cats, wolves, raccoons, genets and hares. Most infected rabbits were rtPCR-positive in skin and not in spleen samples and the opposite was the case for foxes (p < 0.05). L. infantum prevalence was lowest in spring following months of non-exposure to phlebotomine sand fly vectors, and spatially matched recently estimated Phlebotomus perniciosus vector abundance and the prevalence of subclinical infection in dogs and humans. Prevalence increased with altitude and was greater in drier and less windy South and West compared to the coastal Southeast of the study area (p < 0.05). Genetic diversity of L. infantum from foxes, investigated by PCR-restriction fragment length polymorphisms of kinetoplast DNA, revealed B genotype in all animals, which is frequent in people and dogs in the Iberian Peninsula and Morocco. The study provides further evidence that subclinical L. infantum infection is widespread in wildlife with prevalence depending on environmental factors and that parasite tissue tropism may vary according to host species. Moreover, it suggests that sylvatic and domestic transmission cycles are closely interconnected.

KW - Distribution

KW - Genotypes

KW - Leishmania infantum

KW - PCR diagnosis

KW - Wildlife

KW - Spain

U2 - 10.1016/j.vetpar.2018.05.012

DO - 10.1016/j.vetpar.2018.05.012

M3 - Article

VL - Vol. 259

SP - 61

EP - 67

JO - Veterinary Parasitology

JF - Veterinary Parasitology

SN - 0304-4017

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