TY - JOUR
T1 - Ephedra alte extracts' GC-MS profiles and antimicrobial activity against multidrug-resistant pathogens (MRSA)
AU - Salman, Haya Ayyal
AU - Yaakop, Amira Suriaty
AU - Al-Rimawi, Fuad
AU - Makhta, Ana Masara Ahmad
AU - Mousa, Muath
AU - Semreen, Mohammad H.
AU - Alharbi, Naiyf S.
N1 - Funding Information:
This work was supported by the Researchers supporting project number ( RSP2024R70 ), King Saud University , Riyadh, Saudi Arabia.
Funding Information:
This work was supported by the Researchers supporting project number (RSP2024R70), King Saud University, Riyadh, Saudi Arabia.Authors express their sincere appreciation to the Researchers Supporting Project Number (RSP2024R70), King Saud University, Riyadh, Saudi Arabia. The authors acknowledge the School of Biological Sciences, Universiti Sains Malaysia for the facilities provided.
Publisher Copyright:
© 2024
PY - 2024/3/15
Y1 - 2024/3/15
N2 - The extracts of E. alte offer promising potential as renewable resources for various chemical derivative products aimed at addressing antibiotic resistance. These extracts exhibited significant activity against methicillin-resistant Staphylococcus aureus (MRSA), a strain known for its resistance to multiple antibiotics. The extracts were found to be effective against several common antibiotics, including Imipenem, Ampicillin, Penicillin G, Oxacillin, and Amoxicillin-clavulanate. GC-MS analysis revealed that the phytoconstituents of E. alte extracts, obtained using both methanol and ethyl acetate, consist of a diverse range of 83 and 160 phytocompounds, respectively. These organic compounds serve as important biochemical precursors for the synthesis of vitamins E and K1, and exhibit antioxidant, antimicrobial, and anti-inflammatory properties in both plants and microorganisms. Notable compounds identified include fatty acids (such as palmitic acid, dodecanoic acid, sebacic acid, pentadecanoic acid, myristic acid, stearic acid, behenic acid, and linoelaidic acid), phytosterols (Campesterol, β-sitosterol, Stigmast-5-ene), sugars (D-fructose, Fructofuranans), terpenoids (Phytol, citronellol), and phenolic acids (Protocatechoic acid, shikimic acid). The antimicrobial activity of all E. alte extracts was found to be superior to that of mupirocin and ciprofloxacin, as observed in susceptibility testing against MRSA ATCC 43300 and other pathogenic bacteria and fungi. It is likely that the combined action of the antimicrobial components within the E. alte extract bypasses the mechanisms employed by MRSA to protect itself from antibiotics. Further experiments are needed to investigate the individual effects of each pure compound and their potential synergistic interactions, which may enhance their overall performance.
AB - The extracts of E. alte offer promising potential as renewable resources for various chemical derivative products aimed at addressing antibiotic resistance. These extracts exhibited significant activity against methicillin-resistant Staphylococcus aureus (MRSA), a strain known for its resistance to multiple antibiotics. The extracts were found to be effective against several common antibiotics, including Imipenem, Ampicillin, Penicillin G, Oxacillin, and Amoxicillin-clavulanate. GC-MS analysis revealed that the phytoconstituents of E. alte extracts, obtained using both methanol and ethyl acetate, consist of a diverse range of 83 and 160 phytocompounds, respectively. These organic compounds serve as important biochemical precursors for the synthesis of vitamins E and K1, and exhibit antioxidant, antimicrobial, and anti-inflammatory properties in both plants and microorganisms. Notable compounds identified include fatty acids (such as palmitic acid, dodecanoic acid, sebacic acid, pentadecanoic acid, myristic acid, stearic acid, behenic acid, and linoelaidic acid), phytosterols (Campesterol, β-sitosterol, Stigmast-5-ene), sugars (D-fructose, Fructofuranans), terpenoids (Phytol, citronellol), and phenolic acids (Protocatechoic acid, shikimic acid). The antimicrobial activity of all E. alte extracts was found to be superior to that of mupirocin and ciprofloxacin, as observed in susceptibility testing against MRSA ATCC 43300 and other pathogenic bacteria and fungi. It is likely that the combined action of the antimicrobial components within the E. alte extract bypasses the mechanisms employed by MRSA to protect itself from antibiotics. Further experiments are needed to investigate the individual effects of each pure compound and their potential synergistic interactions, which may enhance their overall performance.
KW - Antimicrobial agents
KW - E. alte
KW - GC-MS
KW - HPLC
KW - MRSA
UR - http://www.scopus.com/inward/record.url?scp=85186617769&partnerID=8YFLogxK
U2 - 10.1016/j.heliyon.2024.e27051
DO - 10.1016/j.heliyon.2024.e27051
M3 - Article
C2 - 38444505
AN - SCOPUS:85186617769
SN - 2405-8440
VL - 10
JO - Heliyon
JF - Heliyon
IS - 5
M1 - e27051
ER -
