TY - JOUR
T1 - Enzymatic protein digests do not assist in E. Coli discrimination at the strain level using mass spectrometry
AU - Carreira, Ricardo J.
AU - Nunes-Miranda, J. D.
AU - Gonçalves, Alexandre
AU - Igrejas, Gilberto
AU - Poeta, Patrícia
AU - Gómez-Meire, Silvana
AU - Reboiro-Jato, Miguel
AU - Fdez-Riverola, Florentino
AU - Lodeiro, Carlos
AU - Capelo-Martínez, José Luis
N1 - Gonçalves has a PhD fellowship granted by FCT-Fundação para a Ciência e a Tecnologia and ESF-Eurpoean Social Fund (SFRH/BD/47833/2008).
PY - 2013/6/1
Y1 - 2013/6/1
N2 - Different procedures for matrix assisted laser desorption ionization time of flight mass spectrometry-based E. coli classification at the strain level using the enzymatic digestion of proteins from the cell lysate have been studied. The effects of ultrasonic energy as well as the effects of protein reduction and protein alkylation in the sample treatment and in the subsequent classification were assessed. The final optimal method for classification was then compared with an intact cell-based approach in a different set of samples. Our results show that E. coli classification at the strain level is possible as 12 different strains were correctly classified using intact cell analysis. Overall, the confidence level in classification was higher when the analysis was performed with the intact cell approach.
AB - Different procedures for matrix assisted laser desorption ionization time of flight mass spectrometry-based E. coli classification at the strain level using the enzymatic digestion of proteins from the cell lysate have been studied. The effects of ultrasonic energy as well as the effects of protein reduction and protein alkylation in the sample treatment and in the subsequent classification were assessed. The final optimal method for classification was then compared with an intact cell-based approach in a different set of samples. Our results show that E. coli classification at the strain level is possible as 12 different strains were correctly classified using intact cell analysis. Overall, the confidence level in classification was higher when the analysis was performed with the intact cell approach.
KW - Bacterial
KW - Classification
KW - E. Coli
KW - Fingerprinting
KW - MALDI
UR - http://www.scopus.com/inward/record.url?scp=85038639692&partnerID=8YFLogxK
U2 - 10.5584/jiomics.v3i1.131
DO - 10.5584/jiomics.v3i1.131
M3 - Article
AN - SCOPUS:85038639692
SN - 2182-0287
VL - 3
SP - 44
EP - 50
JO - Journal of Integrated OMICS
JF - Journal of Integrated OMICS
IS - 1
ER -