This work reports the use of the enzyme Pig Liver Esterase (PLE) (EC 188.8.131.52) to catalyse the enantioselective hydrolysis of cis-cyclohex-4-ene-1,2-dicarboxylate (cis-DE) to the chiral product of pharmaceutical interest, methyl-hydrogen (1S-2R)-cyclohex-4-ene-1,2-dicarboxylate ((1S-2R)-ME). For this purpose, a hollow fiber reactor is used, with enzyme immobilized in the membrane porous structure. The membrane module was operated as a contactor between an organic phase, where the substrate is dissolved, and an aqueous phase, to where the product diffuses. The results obtained with immobilized enzyme are compared with those obtained using a bulk biphasic reactor where the aqueous phase with the solubilized enzyme contacts with a non-miscible organic phase containing the substrate.
- Lipase B