Enhanced osteogenesis on biofunctionalized poly(ϵ-caprolactone)/poly(m-anthranilic acid) nanofibers

Zeliha Guler, Jorge C. Silva, Abdulkadir S. Sarac

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Biofunctionalized nanofibers with a desired biological function can be used as a tissue engineering scaffold due to their small fiber diameters and porous structure. In the present study, poly(ϵ-caprolactone)/poly(m-anthranilic acid) nanofibers were biofunctionalized with covalent immobilization of bone morphogenetic protein-2 (BMP-2) through 1-ethyl-3-(dimethyl-aminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide activation. Fourier transform infrared analysis of the nanofiber surfaces confirmed the successful immobilization. The amount of immobilized BMP-2 was determined with bicinchoninic acid protein assay. The nanofibers before and after BMP-2 immobilization were non-cytotoxic and enhanced the attachment and proliferation of Saos-2 cells. Biofunctionalization of nanofibers with BMP-2 promoted in vitro osteogenic activity. The alkaline phosphatase activity and calcium mineralizatio of cells after 14 days of in vitro culture were enhanced on nanofibers with immobilized BMP-2.

Original languageEnglish
Pages (from-to)743-754
Number of pages12
JournalJournal of Biomaterials Applications
Volume31
Issue number5
DOIs
Publication statusPublished - 1 Nov 2016

Keywords

  • biofunctionalization
  • bone morphogenetic protein-2
  • electrospun nanofiber
  • in vitro cell studies
  • Poly (m -antranilic acid)

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