TY - JOUR
T1 - Enhanced osteogenesis on biofunctionalized poly(ϵ-caprolactone)/poly(m-anthranilic acid) nanofibers
AU - Guler, Zeliha
AU - Silva, Jorge C.
AU - Sarac, Abdulkadir S.
N1 - Sem pdf
PY - 2016/11/1
Y1 - 2016/11/1
N2 - Biofunctionalized nanofibers with a desired biological function can be used as a tissue engineering scaffold due to their small fiber diameters and porous structure. In the present study, poly(ϵ-caprolactone)/poly(m-anthranilic acid) nanofibers were biofunctionalized with covalent immobilization of bone morphogenetic protein-2 (BMP-2) through 1-ethyl-3-(dimethyl-aminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide activation. Fourier transform infrared analysis of the nanofiber surfaces confirmed the successful immobilization. The amount of immobilized BMP-2 was determined with bicinchoninic acid protein assay. The nanofibers before and after BMP-2 immobilization were non-cytotoxic and enhanced the attachment and proliferation of Saos-2 cells. Biofunctionalization of nanofibers with BMP-2 promoted in vitro osteogenic activity. The alkaline phosphatase activity and calcium mineralizatio of cells after 14 days of in vitro culture were enhanced on nanofibers with immobilized BMP-2.
AB - Biofunctionalized nanofibers with a desired biological function can be used as a tissue engineering scaffold due to their small fiber diameters and porous structure. In the present study, poly(ϵ-caprolactone)/poly(m-anthranilic acid) nanofibers were biofunctionalized with covalent immobilization of bone morphogenetic protein-2 (BMP-2) through 1-ethyl-3-(dimethyl-aminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide activation. Fourier transform infrared analysis of the nanofiber surfaces confirmed the successful immobilization. The amount of immobilized BMP-2 was determined with bicinchoninic acid protein assay. The nanofibers before and after BMP-2 immobilization were non-cytotoxic and enhanced the attachment and proliferation of Saos-2 cells. Biofunctionalization of nanofibers with BMP-2 promoted in vitro osteogenic activity. The alkaline phosphatase activity and calcium mineralizatio of cells after 14 days of in vitro culture were enhanced on nanofibers with immobilized BMP-2.
KW - biofunctionalization
KW - bone morphogenetic protein-2
KW - electrospun nanofiber
KW - in vitro cell studies
KW - Poly (m -antranilic acid)
UR - http://www.scopus.com/inward/record.url?scp=84994102233&partnerID=8YFLogxK
U2 - 10.1177/0885328216660379
DO - 10.1177/0885328216660379
M3 - Article
AN - SCOPUS:84994102233
SN - 0885-3282
VL - 31
SP - 743
EP - 754
JO - Journal of Biomaterials Applications
JF - Journal of Biomaterials Applications
IS - 5
ER -