Enhanced Direct Electron Transfer of a Multihemic Nitrite Reductase on Single-walled Carbon Nanotube Modified Electrodes

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Abstract

Single-walled carbon nanotubes (SWCNTs) deposits on glassy carbon and pyrolytic graphite electrodes have dramatically enhanced the direct electron transfer of the multihemic nitrite reductase from Desulfovibrio desulfuricans ATCC 27774, enabling a 10-fold increase in catalytic currents. At optimal conditions, the sensitivity to nitrite and the maximum current density were 2.4 +/- 0.1 A L mol(-1) cm(-2) and 1500 mu A cm(-2), respectively. Since the biosensor performance decreased over time, laponite clay and electropolymerized amphiphilic pyrrole were tested as protecting layers. Both coating materials increased substantially the bioelectrode stability, which kept about 90% and 60% of its initial sensitivity to nitrite after 20 and 248 days, respectively.
Original languageUnknown
Pages (from-to)2973-2978
JournalElectroanalysis
Volume22
Issue number24
DOIs
Publication statusPublished - 1 Jan 2010

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