Engineered Protein Variants for Bioconjugation

Cláudia S. M. Fernandes, Gonçalo D.G. Teixeira, Olga Iranzo, Ana C. A. Roque

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

The possibility to covalently modify biological entities, namely proteins, nucleic acids, sugars, is possible through bioconjugation, creating a boundary between biology and chemistry. The field of protein bioconjugation, in particular, has been extremely active and rendered several methods to attain protein conjugates with drugs, enzymes, inorganic or organic materials, dyes and fluorescent reporter molecules, and polymers. The initial bioconjugation methods took advantage of the reactive side chains of naturally occurring amino acids and lately evolved toward the introduction of reactive amino acids at precise locations through DNA recombinant technologies. In addition, unnatural amino acids also provide a source of site-specific and selective bioconjugation tools. Bioconjugation derives from a profound cross talk between disciplines because modern organic chemistry has progressed enormously toward chemoselective reactions performed under physiological conditions.

Original languageEnglish
Title of host publicationBiomedical Applications of Functionalized Nanomaterials: Concepts, Development and Clinical Translation
PublisherElsevier
Pages105-138
Number of pages34
ISBN (Electronic)9780323508797
ISBN (Print)9780323508780
DOIs
Publication statusPublished - 3 Mar 2018

Keywords

  • Alkynes
  • Amino acid
  • Azides
  • Bioconjugation
  • Chemoselective reactions
  • N-hydroxysuccinimide
  • Thiol groups

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    Fernandes, C. S. M., Teixeira, G. D. G., Iranzo, O., & Roque, A. C. A. (2018). Engineered Protein Variants for Bioconjugation. In Biomedical Applications of Functionalized Nanomaterials: Concepts, Development and Clinical Translation (pp. 105-138). Elsevier. https://doi.org/10.1016/B978-0-323-50878-0.00005-7