Engineered Protein Variants for Bioconjugation

Cláudia S. M. Fernandes, Gonçalo D.G. Teixeira, Olga Iranzo, Ana C. A. Roque

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

The possibility to covalently modify biological entities, namely proteins, nucleic acids, sugars, is possible through bioconjugation, creating a boundary between biology and chemistry. The field of protein bioconjugation, in particular, has been extremely active and rendered several methods to attain protein conjugates with drugs, enzymes, inorganic or organic materials, dyes and fluorescent reporter molecules, and polymers. The initial bioconjugation methods took advantage of the reactive side chains of naturally occurring amino acids and lately evolved toward the introduction of reactive amino acids at precise locations through DNA recombinant technologies. In addition, unnatural amino acids also provide a source of site-specific and selective bioconjugation tools. Bioconjugation derives from a profound cross talk between disciplines because modern organic chemistry has progressed enormously toward chemoselective reactions performed under physiological conditions.

Original languageEnglish
Title of host publicationBiomedical Applications of Functionalized Nanomaterials: Concepts, Development and Clinical Translation
PublisherElsevier
Pages105-138
Number of pages34
ISBN (Electronic)9780323508797
ISBN (Print)9780323508780
DOIs
Publication statusPublished - 3 Mar 2018

Fingerprint

Amino acids
Proteins
Amino Acids
Organic Chemistry
Genetic engineering
Recombinant DNA
Nucleic acids
Fluorescent Dyes
Sugars
Nucleic Acids
Polymers
Enzymes
Dyes
Technology
Molecules
Pharmaceutical Preparations

Keywords

  • Alkynes
  • Amino acid
  • Azides
  • Bioconjugation
  • Chemoselective reactions
  • N-hydroxysuccinimide
  • Thiol groups

Cite this

Fernandes, C. S. M., Teixeira, G. D. G., Iranzo, O., & Roque, A. C. A. (2018). Engineered Protein Variants for Bioconjugation. In Biomedical Applications of Functionalized Nanomaterials: Concepts, Development and Clinical Translation (pp. 105-138). Elsevier. https://doi.org/10.1016/B978-0-323-50878-0.00005-7
Fernandes, Cláudia S. M. ; Teixeira, Gonçalo D.G. ; Iranzo, Olga ; Roque, Ana C. A. / Engineered Protein Variants for Bioconjugation. Biomedical Applications of Functionalized Nanomaterials: Concepts, Development and Clinical Translation. Elsevier, 2018. pp. 105-138
@inbook{39eeb79614de48dfb8396427da1e5be2,
title = "Engineered Protein Variants for Bioconjugation",
abstract = "The possibility to covalently modify biological entities, namely proteins, nucleic acids, sugars, is possible through bioconjugation, creating a boundary between biology and chemistry. The field of protein bioconjugation, in particular, has been extremely active and rendered several methods to attain protein conjugates with drugs, enzymes, inorganic or organic materials, dyes and fluorescent reporter molecules, and polymers. The initial bioconjugation methods took advantage of the reactive side chains of naturally occurring amino acids and lately evolved toward the introduction of reactive amino acids at precise locations through DNA recombinant technologies. In addition, unnatural amino acids also provide a source of site-specific and selective bioconjugation tools. Bioconjugation derives from a profound cross talk between disciplines because modern organic chemistry has progressed enormously toward chemoselective reactions performed under physiological conditions.",
keywords = "Alkynes, Amino acid, Azides, Bioconjugation, Chemoselective reactions, N-hydroxysuccinimide, Thiol groups",
author = "Fernandes, {Cl{\'a}udia S. M.} and Teixeira, {Gon{\cc}alo D.G.} and Olga Iranzo and Roque, {Ana C. A.}",
year = "2018",
month = "3",
day = "3",
doi = "10.1016/B978-0-323-50878-0.00005-7",
language = "English",
isbn = "9780323508780",
pages = "105--138",
booktitle = "Biomedical Applications of Functionalized Nanomaterials: Concepts, Development and Clinical Translation",
publisher = "Elsevier",

}

Fernandes, CSM, Teixeira, GDG, Iranzo, O & Roque, ACA 2018, Engineered Protein Variants for Bioconjugation. in Biomedical Applications of Functionalized Nanomaterials: Concepts, Development and Clinical Translation. Elsevier, pp. 105-138. https://doi.org/10.1016/B978-0-323-50878-0.00005-7

Engineered Protein Variants for Bioconjugation. / Fernandes, Cláudia S. M.; Teixeira, Gonçalo D.G.; Iranzo, Olga; Roque, Ana C. A.

Biomedical Applications of Functionalized Nanomaterials: Concepts, Development and Clinical Translation. Elsevier, 2018. p. 105-138.

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Engineered Protein Variants for Bioconjugation

AU - Fernandes, Cláudia S. M.

AU - Teixeira, Gonçalo D.G.

AU - Iranzo, Olga

AU - Roque, Ana C. A.

PY - 2018/3/3

Y1 - 2018/3/3

N2 - The possibility to covalently modify biological entities, namely proteins, nucleic acids, sugars, is possible through bioconjugation, creating a boundary between biology and chemistry. The field of protein bioconjugation, in particular, has been extremely active and rendered several methods to attain protein conjugates with drugs, enzymes, inorganic or organic materials, dyes and fluorescent reporter molecules, and polymers. The initial bioconjugation methods took advantage of the reactive side chains of naturally occurring amino acids and lately evolved toward the introduction of reactive amino acids at precise locations through DNA recombinant technologies. In addition, unnatural amino acids also provide a source of site-specific and selective bioconjugation tools. Bioconjugation derives from a profound cross talk between disciplines because modern organic chemistry has progressed enormously toward chemoselective reactions performed under physiological conditions.

AB - The possibility to covalently modify biological entities, namely proteins, nucleic acids, sugars, is possible through bioconjugation, creating a boundary between biology and chemistry. The field of protein bioconjugation, in particular, has been extremely active and rendered several methods to attain protein conjugates with drugs, enzymes, inorganic or organic materials, dyes and fluorescent reporter molecules, and polymers. The initial bioconjugation methods took advantage of the reactive side chains of naturally occurring amino acids and lately evolved toward the introduction of reactive amino acids at precise locations through DNA recombinant technologies. In addition, unnatural amino acids also provide a source of site-specific and selective bioconjugation tools. Bioconjugation derives from a profound cross talk between disciplines because modern organic chemistry has progressed enormously toward chemoselective reactions performed under physiological conditions.

KW - Alkynes

KW - Amino acid

KW - Azides

KW - Bioconjugation

KW - Chemoselective reactions

KW - N-hydroxysuccinimide

KW - Thiol groups

UR - http://www.scopus.com/inward/record.url?scp=85054650888&partnerID=8YFLogxK

U2 - 10.1016/B978-0-323-50878-0.00005-7

DO - 10.1016/B978-0-323-50878-0.00005-7

M3 - Chapter

SN - 9780323508780

SP - 105

EP - 138

BT - Biomedical Applications of Functionalized Nanomaterials: Concepts, Development and Clinical Translation

PB - Elsevier

ER -

Fernandes CSM, Teixeira GDG, Iranzo O, Roque ACA. Engineered Protein Variants for Bioconjugation. In Biomedical Applications of Functionalized Nanomaterials: Concepts, Development and Clinical Translation. Elsevier. 2018. p. 105-138 https://doi.org/10.1016/B978-0-323-50878-0.00005-7