TY - JOUR
T1 - Energy dispersive X-ray fluorescence quantitative analysis of biological samples with the external standard method
AU - Carvalho, Patrícia M.S.
AU - Pessanha, Sofia
AU - Machado, Jorge
AU - Silva, Ana Luísa
AU - Veloso, João F. C. A.
AU - Casal, Diogo
AU - Pais, Diogo
AU - Santos, José Paulo
N1 - PTDC/FIS-AQM/32536/2017 through FEDER and Fundacao para a CiEncia e Tecnologia (FCT) (Portugal)
UID/FIS/04559/2019 to LIBPhys-UNL from the FCT/MCTES/PIDDAC, Portugal.
Patricia M. S. Carvalho acknowledges the support of FCT (Portugal) under contract No. PD/BD/128324/2017.
PY - 2020/12
Y1 - 2020/12
N2 - Trace elements are present in minute amounts in the human body but contribute to its proper functioning, by participating in several biological processes. Imbalance of the concentrations of these elements can lead to the development of pathologies, including cancer. As such, the determination of trace element content in tumour tissues and its comparison with normal ones may be helpful for a better understanding of carcinogenesis. In this work, we address the collection and preparation of biological samples for Energy Dispersive X-Ray Fluorescence (EDXRF) analysis, and present a model for the quantification of trace elements, based on the external standard method of quantification. The model was used for the quantification of iron, copper, and zinc in a set of paired samples (normal and tumour tissues). The obtained results show the validity of the method and variations of the elemental concentrations in the different tissues.
AB - Trace elements are present in minute amounts in the human body but contribute to its proper functioning, by participating in several biological processes. Imbalance of the concentrations of these elements can lead to the development of pathologies, including cancer. As such, the determination of trace element content in tumour tissues and its comparison with normal ones may be helpful for a better understanding of carcinogenesis. In this work, we address the collection and preparation of biological samples for Energy Dispersive X-Ray Fluorescence (EDXRF) analysis, and present a model for the quantification of trace elements, based on the external standard method of quantification. The model was used for the quantification of iron, copper, and zinc in a set of paired samples (normal and tumour tissues). The obtained results show the validity of the method and variations of the elemental concentrations in the different tissues.
KW - Cancer
KW - Energy dispersive X-ray fluorescence
KW - External standard method
KW - Quantitative analysis
KW - Trace elements
UR - https://www.scopus.com/pages/publications/85092457174
U2 - 10.1016/j.sab.2020.105991
DO - 10.1016/j.sab.2020.105991
M3 - Article
AN - SCOPUS:85092457174
SN - 0584-8547
VL - 174
JO - Spectrochimica Acta Part B: Atomic Spectroscopy
JF - Spectrochimica Acta Part B: Atomic Spectroscopy
M1 - 105991
ER -
